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Anti-UBE2E2 (Polyclonal), ALEXA Fluor 594

Anti-UBE2E2 (Polyclonal), ALEXA Fluor 594 size: 100 microliters 489

Supplier BIOSS POLYCLONAL ANTIBODIES
Price 489
Size 100 microliters
Contact us Ask Component cell markers and DNA bp. markers are genetic markerser genes or DNA sequenceswith selection audit
TypeConjugated Primary Antibody
Conjugated with 594, ALEXA FLUOR®
Host organismRabbit (Oryctolagus cuniculus)
Target Protein/PeptideUBE2E2
SpecificityThis antibody reacts specifically with UBE2E2
ModificationNo modification has been applied to this antibody
Modification siteNone
ClonalityPolyclonal Antibody
ClonePolyclonal Antibodies
Concentration1ug per 1ul
Subcellular locationsN/A
Antigen SourceKLH conjugated synthetic peptide derived from human UBE2E2
Gene ID7325
Swiss ProtN/A
ApplicationsIF(IHC-P)
Applications with corresponding dilutionsIF(IHC-P)(1:50-200)
Cross reactive species Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens)
Cross Reactive Species details However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species
Background information A wide range of enzymes facilitate this proteolytic ubiquitin pathway, The E3 enzyme then catalyzes the transfer of ubiquitin from E2 to the appropriate protein substrate, The first step in the ubiquitination process requires the ATP-dependent activation of the ubiquitin C-terminus and the assembly of multi-ubiquitin chains by the E1 enzyme, The three classes of enzymes involved in ubiquitination are the ubiquitin-activating enzymes (E1s), The ubiquitin chain is then conjugated to the E2 enzyme to generate an intermediate ubiquitin-E2 complex, one of which is UBE2E2 (also known as UBCH8 in human), the ubiquitin-conjugating enzymes (E2s) and the ubiquitin-protein ligases (E3s), thereby playing an important role in protein degradation, thereby targeting that substrate for degradation, which functions as an E2 enzyme and catalyzes the ATP-dependent covalent attachment of ubiquitin to target proteins, Ubiquitination is an important mechanism through which three classes of enzymes act in concert to target short-lived or abnormal proteins for destruction
Purification methodPurified by Protein A
Storage 50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA
Excitation emission590nm/617nm
Synonyms UbcH8 FLJ25157
Also known asUBE2E2 Polyclonal Antibody
Other nameAnti-UBE2E2 Polyclonal
Advisory For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody
PropertiesFor facs or microscopy Alexa 1 conjugate
ConjugationAlexa Fluor
GroupPolyclonals and antibodies
About The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot
Gene targetUBE2E2
Short nameAnti-UBE2E2 (Polyclonal) Fluor 594
Technique Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different 
LabelALEXA
Alternative name ALEXA Fluor 594, antibody to-UBE2E2 (polyclonal)
Alternative techniquepolyclonals
Identity 12478
Gene UBE2E2
Long gene name ubiquitin conjugating enzyme E2 E2
Synonyms gene name ubiquitin-conjugating enzyme E2E 2 (homologous to yeast UBC4/5) ubiquitin-conjugating enzyme E2E 2 (UBC4/5 homolog, yeast) ubiquitin-conjugating enzyme E2E 2
Locus 3p24, 3
Discovery year 1997-12-15
GenBank acession AK057886
Entrez gene record 7325
Pubmed identfication 9371400
RefSeq identity NM_152653
Classification Ubiquitin conjugating enzymes E2
Havana BLAST/BLAT OTTHUMG00000130482

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