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Anti-UBE2U (Polyclonal), ALEXA Fluor 594

Anti-UBE2U (Polyclonal), ALEXA Fluor 594 size: 100 microliters 489

Price 489
Size 100 microliters
TypeConjugated Primary Antibody
Conjugated with 594, ALEXA FLUOR®
Host organismRabbit (Oryctolagus cuniculus)
Target Protein/PeptideUBE2U
SpecificityThis antibody reacts specifically with UBE2U
ModificationNo modification has been applied to this antibody
Modification siteNone
ClonalityPolyclonal Antibody
ClonePolyclonal Antibodies
Concentration1ug per 1ul
Subcellular locationsN/A
Antigen SourceKLH conjugated synthetic peptide derived from human UBE2U
Gene ID148581
Swiss ProtN/A
ApplicationsIF(IHC-P)
Applications with corresponding dilutionsIF(IHC-P)(1:50-200)
Cross reactive speciesHuman (Homo sapiens)
Cross Reactive Species details However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species
Background information Existing as two alternatively spliced isoforms, The Ub chain is then conjugated to the Ub-conjugating enzyme (E2) to generate an intermediate Ub-E2 complex, The Ub-ligase (E3) then catalyzes the transfer of Ub from E2 to the appropriate protein substrate, The first step requires ATP-dependent activation of the Ub C-terminus and assembly of multi-Ub chains by a Ub-activating enzyme known as the E1 component, UBE2U (ubiquitin-conjugating enzyme E2U) is a 321 amino acid E2 ubiquitin conjugating enzyme that catalyzes the covalent attachment of ubiquitin to other proteins, UBE2U is encoded by a gene located on human chromosome 1, contains over 3, which spans 260 million base pairs, 000 genes and comprises nearly 8% of the human genome, The ubiquitin (Ub) pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub and Ub-like molecules to specific protein substrates
Purification methodPurified by Protein A
Storage 50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA
Excitation emission590nm/617nm
Synonyms MGC35130
Also known asUBE2U Polyclonal Antibody
Other nameAnti-UBE2U Polyclonal
Advisory For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody
PropertiesFor facs or microscopy Alexa 1 conjugate
ConjugationAlexa Fluor
GroupPolyclonals and antibodies
About The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot
Gene targetUBE2U
Short nameAnti-UBE2U (Polyclonal) Fluor 594
Technique Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different 
LabelALEXA
Alternative name ALEXA Fluor 594, antibody to-UBE2U (polyclonal)
Alternative techniquepolyclonals
Identity 28559
Gene UBE2U
Long gene name ubiquitin conjugating enzyme E2 U (putative)
Synonyms gene name ubiquitin-conjugating enzyme E2U (putative)
Locus 1p31, 3
Discovery year 2005-03-21
GenBank acession BC029895
Entrez gene record 148581
Pubmed identfication 12477932
RefSeq identity NM_152489
Classification Ubiquitin conjugating enzymes E2
Havana BLAST/BLAT OTTHUMG00000009023

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