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Anti-UBE3A/E6-AP (Polyclonal), ALEXA Fluor 594

Anti-UBE3A/E6-AP (Polyclonal), ALEXA Fluor 594 size: 100 microliters 489

Supplier BIOSS POLYCLONAL ANTIBODIES
Price 489
Size 100 microliters
Contact us Ask Component cell markers and DNA bp. markers are genetic markerser genes or DNA sequenceswith selection audit
TypeConjugated Primary Antibody
Conjugated with 594, ALEXA FLUOR®
Host organismRabbit (Oryctolagus cuniculus)
Target Protein/PeptideUBE3A/E6-AP
SpecificityThis antibody reacts specifically with UBE3A/E6-AP
ModificationNo modification has been applied to this antibody
Modification siteNone
ClonalityPolyclonal Antibody
ClonePolyclonal Antibodies
Concentration1ug per 1ul
Subcellular locationsN/A
Antigen SourceKLH conjugated synthetic peptide derived from human UBE3A/E6-AP
Gene ID7337
Swiss ProtN/A
ApplicationsIF(IHC-P)
Applications with corresponding dilutionsIF(IHC-P)(1:50-200)
Cross reactive species Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens)
Cross Reactive Species details However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species
Background information Genetic mutations that impair E6-AP activity result in the accumulation of p53 in the cytoplasm, HHR23A, Previous studies have indicated that E6-AP associates with the human papillomavirus E6 oncogene, Several substrates of E6-AP have been identified and include the tumor suppressor protein p53 and the mammalian homolog of Rad23, This conjugation is facilitated by the enzymatic activity of E3 ubiquitin ligase family members such as E6-AP, Ubiquitin (Ub) is directly conjugated to protein substrates by the transfer of Ub from an E2 ubiquitin conjugating enzyme to the target protein, and, in many instances, these mutations are associated with the development of the rare neurodevelopmental disorder Angelman syndrome (AS), which complexes with p53 and thereby potentiates E6-AP mediated ubiquitination of p53, which is characterized by severe motor dysfunction and mental retardation, which selectively targets proteins for degradation by the 26S proteasome, E6-associating protein is a component of the ubiquitin-mediated proteolytic pathway
Purification methodPurified by Protein A
Storage 50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA
Excitation emission590nm/617nm
Synonyms AS ANCR E6-AP FLJ26981
Also known asUBE3A/E6-AP Polyclonal Antibody
Other nameAnti-UBE3A/E6-AP Polyclonal
Advisory For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody
PropertiesFor facs or microscopy Alexa 1 conjugate
ConjugationAlexa Fluor
GroupPolyclonals and antibodies
About The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot
Gene targetUBE3A/E6-AP
Short nameAnti-UBE3A/E6-AP (Polyclonal) Fluor 594
Technique Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different 
LabelAP
Alternative name ALEXA Fluor 594, antibody to-ubiquitin protein ligase E3A/E6-AP (polyclonal)
Alternative techniquepolyclonals
Alternative to gene target ANCR and AS and E6-AP and EPVE6AP and HPVE6A, UBE3A and IDBG-4274 and ENSG00000114062 and 7337, UBE3A and IDBG-628537 and ENSBTAG00000002487 and 100335247, Ube3a and IDBG-190437 and ENSMUSG00000025326 and 22215, ligase activity, nuclei, this GO :0000502 and proteasome complex and cellular component this GO :0001541 and ovarian follicle development and biological process this GO :0003713 and transcription coactivator activity and molecular function this GO :0004842 and ubiquitin-protein transferase activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0005829 and cytosol and cellular component this GO :0006508 and proteolysis and biological process this GO :0006511 and ubiquitin-dependent protein catabolic process and biological process this GO :0007420 and brain development and biological process this GO :0014068 and positive regulation of phosphatidylinositol 3-kinase signaling and biological process this GO :0016032 and viral process and biological process this GO :0016567 and protein ubiquitination and biological process this GO :0016874 and ligase activity and molecular function this GO :0030521 and androgen receptor signaling pathway and biological process this GO :0035037 and sperm entry and biological process this GO :0042787 and protein ubiquitination involved in ubiquitin-dependent protein catabolic process and biological process this GO :0045944 and positive regulation of transcription from RNA polymerase II promoter and biological process this GO :0051865 and protein autoubiquitination and biological process this GO :0060736 and prostate gland growth and biological process this GO :0070936 and protein K48-linked ubiquitination and biological process, this GO :0003713: transcription coactivator activity, this GO :0003713: transcription coactivator activity and also this GO :0004842: ubiquitin-protein transferase activity and also this GO :0005515: protein binding and also this GO :0016874: ligase activity, this GO :0004842: ubiquitin-protein transferase activity, this GO :0005515: protein binding, this GO :0016874: ligase activity, 533136, ubiquitin protein ligase E3A
Identity 12496
Gene UBE3A
Long gene name ubiquitin protein ligase E3A
Synonyms gene EPVE6AP HPVE6A
Synonyms gene name human papilloma virus E6-associated protein
Synonyms name Angelman syndrome
Locus 15q11, 2
Discovery year 1993-10-21
GenBank acession AF002224
Entrez gene record 7337
Pubmed identfication 8221889
RefSeq identity NM_000462
Havana BLAST/BLAT OTTHUMG00000171548
Locus Specific Databases Manchester LOVD LRG_15 , NGRL

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