Name : Anti-VGLL4 (Polyclonal), ALEXA Fluor 594
Supplier : BIOSS POLYCLONAL ANTIBODIES
Price :489
SKU : GEN3830804128
| Type | Conjugated Primary Antibody |
| Conjugated with | 594, ALEXA FLUOR® |
| Host organism | Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide | VGLL4 |
| Specificity | This antibody reacts specifically with VGLL4 |
| Modification | No modification has been applied to this antibody |
| Modification site | None |
| Clonality | Polyclonal Antibody |
| Clone | Polyclonal Antibodies |
| Concentration | 1ug per 1ul |
| Subcellular locations | N/A |
| Antigen Source | KLH conjugated synthetic peptide derived from human VGLL4 |
| Gene ID | 9686 |
| Swiss Prot | N/A |
| Applications | IF(IHC-P) |
| Applications with corresponding dilutions | IF(IHC-P)(1:50-200) |
| Cross reactive species | Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details | However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information | Overexpression of Vgl-4 in cardiac myocytes interferes with basal expression and ?-adrenergic receptor-dependent activation of a TEF-1 dependent skeletal ?actin promoter, The mechanism whereby TEF-1 confers tissue specificity depends largely on the interaction of TEF-1 with tissue-specific cofactors, There are two isoforms of Vgl-4 that are produced as a result of alternative splicing events, This suggests that Vgl-4 counteracts ?-adrenergic activation of gene expression in cardiomyocytes, Transcription cofactor Vgl-4 (vestigial-like protein 4) is a 290 amino acid nuclear protein that interacts with TEF-1 and MEF-2, Vgl-4 is the only member of the vestigial-like family that is expressed in heart, The transcriptional enhancer factor-1 (TEF-1) family of transcription factors regulate tissue-specific gene expression in muscle and placenta |
| Purification method | Purified by Protein A |
| Storage | 50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission | 590nm/617nm |
| Synonyms | KIAA0121 |
| Also known as | VGLL4 Polyclonal Antibody |
| Other name | Anti-VGLL4 Polyclonal |
| Advisory | For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties | For facs or microscopy Alexa 1 conjugate |
| Conjugation | Alexa Fluor |
| Group | Polyclonals and antibodies |
| About | The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target | VGLL4 |
| Short name | Anti-VGLL4 (Polyclonal) Fluor 594 |
| Technique | Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different  |
| Label | ALEXA |
| Alternative name | ALEXA Fluor 594, antibody to-VGLL4 (polyclonal) |
| Alternative technique | polyclonals |
| Identity | 28966 |
| Gene | VGLL4 |
| Long gene name | vestigial like family member 4 |
| Synonyms gene name | vestigial like 4 (Drosophila) vestigial-like family member 4 |
| Locus | 3p25, 2 , 3-p25 |
| Discovery year | 2004-06-03 |
| GenBank acession | D50911 |
| Entrez gene record | 9686 |
| Pubmed identfication | 8590280 15140898 |
| RefSeq identity | NM_014667 |
| Classification | Vestigial like family |
| Havana BLAST/BLAT | OTTHUMG00000129739 |