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ARFGEF2/BIG2 Antibody, FITC Conjugated

ARFGEF2/BIG2 Antibody, FITC Conjugated size: 0.1ml 333

Supplier BIOSS PRIMARY CONJUGATED ANTIBODIES 1
Price 333
Size 0.1ml
Contact us Ask Component cell markers and DNA bp. markers are genetic markerser genes or DNA sequenceswith selection audit
Long name FITC Conjugated, ARFGEF2/BIG2 Polyclonal Antibody
CategoryConjugated Primary Antibodies
ConjugationAnti-FITC Antibody
Host OrganismRabbit (Oryctolagus cuniculus)
Target AntigenARFGEF2/BIG2
SpecificityThis is a highly specific antibody against ARFGEF2/BIG2
ModificationUnmodified
Modification siteNone
ClonalityPolyclonal
ClonePolyclonal antibody
Concentration1ug per 1ul
SourceKLH conjugated synthetic peptide derived from human ARFGEF2/BIG2
Tested applicationsIF(IHC-P)
Recommended dilutionsIF(IHC-P)(1:50-200)
Crossreactivity Mouse, Rat, Human
Crossreactive species details not every possible cross-reactivity is known, Due to limited amount of testing and knowledge
Antigen background BIG1 and BIG2 exist in macromolecular complexes that move between the golgi membranes and cytosol, BIG2 associates with PKA regulatory subunits, Both GEPS contain a sec7 domain that is responsible for their brefeldin inhibition and also their catalytic activity, GEPs comprise two major families, In vivo, Two genes for GEPs found on human chromosomes 8 and 20 encode BFA sensitive GEPs designated BIG1 and BIG2, a family of guanine nucleotide-binding proteins that play an important role in intracellular vesicular trafficking, a protein that effects golgi structure, and a group of smaller GEPs that are insenstive to BFA, implying that BIG2 may act as an A kinase-anchoring protein (AKAP) that could coordinate the cAMP and ARF regulatory pathways, large GEPs that are inhibited by brefeldin A (BFA), Guanine nucleotide-exchange proteins (GEPs) accelerate replacement of bound GDP with GTP and thereby activate ADP-ribosylation factors (ARFs)
Purification methodThis antibody was purified via Protein A
Storage conditions 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA
Excitation Emission494nm/518nm
Synonyms BIG2
Properties Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°
Gene targetARFGEF2/BIG2 Conjugated
Short name FITC Conjugated, ARFGEF2/BIG2 Antibody
Technique Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody
LabelFITC
Alternative name fluorecein coupled, ADP-ribosylation factor guanine nucleotide-exchange factor 2 (brefeldin A-inhibited)/BIG2 (Antibody to)
Alternative techniqueantibodies
Alternative to gene target ARFGEF2 and IDBG-643137 and ENSBTAG00000007120 and 539295, ARFGEF2 and IDBG-80059 and ENSG00000124198 and 10564, Arfgef2 and IDBG-212930 and ENSMUSG00000074582 and 99371, BIG2 and dJ1164I10, Cell surfaces, GABA receptor binding, this GO :0000139 and this GO lgi membrane and cellular component this GO :0001881 and receptor recycling and biological process this GO :0005085 and guanyl-nucleotide exchange factor activity and molecular function this GO :0005086 and ARF guanyl-nucleotide exchange factor activity and molecular function this GO :0005488 and binding and molecular function this GO :0005515 and protein binding and molecular function this GO :0005737 and cytoplasm and cellular component this GO :0005802 and trans- this GO lgi network and cellular component this GO :0005815 and microtubule organizing center and cellular component this GO :0005829 and cytosol and cellular component this GO :0005879 and axonemal microtubule and cellular component this GO :0006887 and exocytosis and biological process this GO :0006893 and this GO lgi to plasma membrane transport and biological process this GO :0007032 and endosome organization and biological process this GO :0010256 and endomembrane system organization and biological process this GO :0015031 and protein transport and biological process this GO :0016020 and membrane and cellular component this GO :0016023 and cytoplasmic membrane-bounded vesicle and cellular component this GO :0016192 and vesicle-mediated transport and biological process this GO :0017022 and myosin binding and molecular function this GO :0030054 and cell junction and cellular component this GO :0031410 and cytoplasmic vesicle and cellular component this GO :0032012 and regulation of ARF protein signal transduction and biological process this GO :0032279 and asymmetric synapse and cellular component this GO :0032280 and symmetric synapse and cellular component this GO :0032760 and positive regulation of tumor necrosis factor production and biological process this GO :0034237 and protein kinase A regulatory subunit binding and molecular function this GO :0035556 and intracellular signal transduction and biological process this GO :0043197 and dendritic spine and cellular component this GO :0043547 and positive regulation of GTPase activity and biological process this GO :0048471 and perinuclear region of cytoplasm and cellular component this GO :0050811 and GABA receptor binding and molecular function this GO :0055037 and recycling endosome and cellular component, this GO :0005085: guanyl-nucleotide exchange factor activity, this GO :0005085: guanyl-nucleotide exchange factor activity and also this GO :0005086: ARF guanyl-nucleotide exchange factor activity and also this GO :0005488: binding and also this GO :0005515: protein binding and also this GO :0017022: myosin binding and also this GO :0034237: protein kinase A regulatory subunit binding and also this GO :0050811: GABA receptor binding, this GO :0005086: ARF guanyl-nucleotide exchange factor activity, this GO :0005488: binding, this GO :0005515: protein binding, this GO :0017022: myosin binding, this GO :0034237: protein kinase A regulatory subunit binding, this GO :0050811: GABA receptor binding, 1 and PVNH2, ADP-ribosylation factor guanine nucleotide-exchange factor 2 (brefeldin A-inhibited)
Identity 15853
Gene ARFGEF2
Long gene name ADP ribosylation factor guanine nucleotide exchange factor 2
Synonyms gene name ADP-ribosylation factor guanine nucleotide-exchange factor 2 (brefeldin A-inhibited)
Synonyms name Brefeldin A-inhibited guanine nucleotide-exchange protein 2
Locus 20q13, 13
Discovery year 2001-06-21
GenBank acession AF084521
Entrez gene record 10564
Pubmed identfication 10212200
RefSeq identity NM_006420
Classification A-kinase anchoring proteins
Havana BLAST/BLAT OTTHUMG00000032687

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