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ASK1 (Ser83) Antibody, FITC Conjugated

ASK1 (Ser83) Antibody, FITC Conjugated size: 0.1ml 350

Supplier BIOSS PRIMARY CONJUGATED ANTIBODIES 1
Price 350
Size 0.1ml
Contact us Ask Component cell markers and DNA bp. markers are genetic markerser genes or DNA sequenceswith selection audit
Long name FITC Conjugated, ASK1 (Ser83) Polyclonal Antibody
CategoryConjugated Primary Antibodies
ConjugationAnti-FITC Antibody
Host OrganismRabbit (Oryctolagus cuniculus)
Target AntigenASK1 Ser83
SpecificityThis is a highly specific antibody against ASK1 Ser83
ModificationPhosphorylation
Modification siteSer83
ClonalityPolyclonal
ClonePolyclonal antibody
Concentration1ug per 1ul
SourceKLH conjugated synthetic phosphopeptide derived from human ASK1 around the phosphorylation site of Ser83
Gene ID number4217
Tested applicationsIF(IHC-P)
Recommended dilutionsIF(IHC-P)(1:50-200)
CrossreactivityHuman
Crossreactive species details not every possible cross-reactivity is known, Due to limited amount of testing and knowledge
Antigen background Drosophila, MAPK kinase (MKK or MEK), MAPK kinase/MEK, MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPKK4) in vitro, MAPKKK5 contains 1, MAPKKK5 does not activate MAPK/ERK, Northern blot analysis shows that MAPKKK5 transcript is abundantly expressed in human heart and pancreas, The MAPKKK5 protein phosphorylates and activates MKK4 (aliases SERK1, The kinases of these signaling cascades are highly conserved, and MAPK kinase kinase (MAPKKK or MEKK), and activates c-Jun N-terminal kinase (JNK)/stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells, and homologs exist in yeast, and mammalian cells, which in turn activates MAPK, 374 amino acids with all 11 kinase subdomains, Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK)
Purification methodThis antibody was purified via Protein A
Storage conditions 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA
Excitation Emission494nm/518nm
Synonyms MAPKKK5 ASK1
Properties Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°
Gene targetASK1 (Ser83) Conjugated
Short name FITC Conjugated, ASK1 (Ser83) Antibody
Technique Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody
LabelFITC
Alternative name fluorecein coupled, ASK1 (Ser83) (Antibody to)
Alternative techniqueantibodies
Identity 6857
Gene MAP3K5
Long gene name mitogen-activated protein kinase kinase kinase 5
Synonyms gene MEKK5
Synonyms name apoptosis signal regulating kinase 1
Locus 6q23, 3
Discovery year 1997-11-14
GenBank acession U67156
Entrez gene record 4217
Pubmed identfication 9465908
Classification Mitogen-activated protein kinase kinase kinases
Havana BLAST/BLAT OTTHUMG00000015647
Locus Specific Databases The Globin Gene Server

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