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ATF2 (Thr51 + Thr53) Antibody, FITC Conjugated

ATF2 (Thr51 + Thr53) Antibody, FITC Conjugated size: 0.1ml 350

Supplier BIOSS PRIMARY CONJUGATED ANTIBODIES 1
Price 350
Size 0.1ml
Contact us Ask Component cell markers and DNA bp. markers are genetic markerser genes or DNA sequenceswith selection audit
Long name FITC Conjugated, ATF2 (Thr51 + Thr53) Polyclonal Antibody
CategoryConjugated Primary Antibodies
ConjugationAnti-FITC Antibody
Host OrganismRabbit (Oryctolagus cuniculus)
Target AntigenATF2 Thr51 + Thr53
SpecificityThis is a highly specific antibody against ATF2 Thr51 + Thr53
ModificationPhosphorylation
Modification siteThr51 + Thr53
ClonalityPolyclonal
ClonePolyclonal antibody
Concentration1ug per 1ul
SourceKLH conjugated synthetic phosphopeptide derived from mouse ATF2 around the phosphorylation site of Thr51 + Thr53
Gene ID number11909
Tested applicationsIF(IHC-P)
Recommended dilutionsIF(IHC-P)(1:50-200)
Crossreactivity Mouse, Rat, Human
Crossreactive species details not every possible cross-reactivity is known, Due to limited amount of testing and knowledge
Antigen background ATF2 is a nuclear protein that binds DNA as a dimer and can form dimers with members of the ATF/CREB and Jun/Fos families, ATF2 is abundantly expressed in brain, Following dimerization ATF2 becomes a short lived protein that undergoes ubiquitination and proteolysis, It is a stronger activator as a heterodimer with cJun than as a homodimer, Many of these genes are important in cell growth and differentiation, Several isoforms of ATF2 arise by differential splicing, Stimulation of the transcriptional activity of ATF2 occurs following cellular stress induced by several genotoxic agents, The stable native full length ATF2 is transcriptionally inactive as a result of an inhibitory direct intramolecular interaction of its carboxy terminal DNA binding domain with the amino terminal transactivation domain, This activation requires phosphorylation of two threonine residues in ATF2 by both JNK/SAP kinase and p38 MAP kinase, and UV irradiation, and in stress and immune responses, inflammatory cytokines, seemingly in a protein phosphatase-dependent mechanism, ATF2 is a member of the ATF/CREB family of basic region leucine zipper DNA binding proteins that regulates transcription by binding to a consensus cAMP response element (CRE) in the promoter of various viral and cellular genes
Purification methodThis antibody was purified via Protein A
Storage conditions 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA
Excitation Emission494nm/518nm
Synonyms ATF1 ATF2 HFB1-GDNF
Properties Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°
Gene targetATF2 (Thr51 + Thr53) Conjugated
Short name FITC Conjugated, ATF2 (Thr51 + Thr53) Antibody
Technique Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody
LabelFITC
Alternative name fluorecein coupled, ATF2 (Thr51 and Thr53) (Antibody to)
Alternative techniqueantibodies
Identity 4232
Gene GDNF
Long gene name glial cell derived neurotrophic factor
Synonyms name astrocyte-derived trophic factor glial cell line derived neurotrophic factor glial derived neurotrophic factor
Locus 5p13, 2
Discovery year 1995-05-04
Entrez gene record 2668
Pubmed identfication 8493557
RefSeq identity NM_000514
Classification Endogenous ligands
Havana BLAST/BLAT OTTHUMG00000090809

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