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ATG1/ULK1 Antibody, FITC Conjugated

ATG1/ULK1 Antibody, FITC Conjugated size: 0.1ml 333

Supplier BIOSS PRIMARY CONJUGATED ANTIBODIES 1
Price 333
Size 0.1ml
Contact us Ask Component cell markers and DNA bp. markers are genetic markerser genes or DNA sequenceswith selection audit
Long name FITC Conjugated, ATG1/ULK1 Polyclonal Antibody
CategoryConjugated Primary Antibodies
ConjugationAnti-FITC Antibody
Host OrganismRabbit (Oryctolagus cuniculus)
Target AntigenATG1/ULK1
SpecificityThis is a highly specific antibody against ATG1/ULK1
ModificationUnmodified
Modification siteNone
ClonalityPolyclonal
ClonePolyclonal antibody
Concentration1ug per 1ul
Immunogen_range1004-1050/1050
Subcellular locationCytoplasm
SourceKLH conjugated synthetic peptide derived from human ATG1/ULK1
Gene ID number8408
Swiss ProtO75385
Tested applicationsIF(IHC-P)
Recommended dilutionsIF(IHC-P)(1:50-200)
Crossreactivity Mouse, Rat, Human
Crossreactive species details not every possible cross-reactivity is known, Due to limited amount of testing and knowledge
Antigen background Activated via phosphorylation by AMPK and also acts as a regulator of AMPK by mediating phosphorylation of AMPK subunits PRKAA1, Acts upstream of phosphatidylinositol 3-kinase PIK3C3 to regulate the formation of autophagophores, May phosphorylate ATG13/KIAA652 and RPTOR, PRKAB2 and PRKAG1, Part of regulatory feedback loops in autophagy: acts both as a downstream effector and negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR, Plays a role early in neuronal differentiation and is required for granule cell axon formation, however such data need additional evidences, leading to negatively regulate AMPK activity, the precursors of autophagosomes, Serine/threonine-protein kinase involved in autophagy in response to starvation
Purification methodThis antibody was purified via Protein A
Storage conditions 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA
Excitation Emission494nm/518nm
Synonyms ATG1 ATG1A
Properties Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°
Gene targetATG1/ULK1 Conjugated
Short name FITC Conjugated, ATG1/ULK1 Antibody
Technique Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody
LabelFITC
Alternative name fluorecein coupled, ATG1/ULK1 (Antibody to)
Alternative techniqueantibodies
Identity 12558
Gene ULK1
Long gene name unc-51 like autophagy activating kinase 1
Synonyms gene name elegans) , elegans)-like kinase 1 unc-51-like kinase 1 (C, unc-51 (C
Synonyms name ATG1 autophagy related 1 homolog (S, cerevisiae)
Locus 12q24, 33
Discovery year 1998-09-03
GenBank acession AF045458
Entrez gene record 8408
Pubmed identfication 9693035
Classification Autophagy related
Havana BLAST/BLAT OTTHUMG00000168052

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