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ATG3 Antibody, Biotin Conjugated

ATG3 Antibody, Biotin Conjugated size: 0.1ml 333

Supplier BIOSS PRIMARY CONJUGATED ANTIBODIES 1
Price 333
Size 0.1ml
Contact us Ask Component cell markers and DNA bp. markers are genetic markerser genes or DNA sequenceswith selection audit
Long name Biotin Conjugated, ATG3 Polyclonal Antibody
CategoryConjugated Primary Antibodies
ConjugationBiotinylated
Host OrganismRabbit (Oryctolagus cuniculus)
Target AntigenATG3
SpecificityThis is a highly specific antibody against ATG3
ModificationUnmodified
Modification siteNone
ClonalityPolyclonal
ClonePolyclonal antibody
Concentration1ug per 1ul
Immunogen_range50-100/314
Subcellular locationCytoplasm
SourceKLH conjugated synthetic peptide derived from human ATG3
Gene ID number64422
Swiss ProtQ9NT62
Tested applications IHC-P, WB
Recommended dilutions IHC-P(1:100-500), WB(1:100-1000)
Crossreactivity Mouse, Rat, Human
Crossreactive species details not every possible cross-reactivity is known, Due to limited amount of testing and knowledge
Antigen background ATG12 conjugation to ATG3 playing a role in mitochondrial homeostasis but not in autophagy, ATG7 (E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with ATG3, Also acts as an autocatalytic E2-like enzyme, GABARAPL1, GABARAPL2 or MAP1LC3A), Preferred substrate is MAP1LC3A, Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway, Responsible for the E2-like covalent binding of phosphatidylethanolamine to the C-terminal Gly of ATG8-like proteins (GABARAP, The ATG12-ATG5 conjugate plays a role of an E3 and promotes the transfer of ATG8-like proteins from ATG3 to phosphatidylethanolamine (PE), The formation of the ATG8-phosphatidylethanolamine conjugates is essential for autophagy and for the cytoplasm to vacuole transport (Cvt), This step is required for the membrane association of ATG8-like proteins, and mitochondrial homeostasis, autophagy, catalyzing the conjugation of ATG12 to itself, E2 conjugating enzyme required for the cytoplasm to vacuole transport (Cvt)
Purification methodThis antibody was purified via Protein A
Storage conditions 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA
Excitation EmissionN/A
Synonyms PC3-96 FLJ22125 MGC15201 DKFZp564M1178
Properties Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, Biotin conjugates can be detected by horseradish peroxidase, To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, alkaline phosphatase substrates or anti biotin conjugated antibodies, 6, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°
Gene targetATG3 Conjugated
Short name Conjugated, ATG3 Antibody
Technique antibodies against human proteins, antibodies for, antibody Conjugates, Antibody
LabelBiotin
Alternative name biotinilated coupled, autophagy related 3 (Antibody to)
Alternative techniqueantibodies
Alternative to gene target ATG3 and IDBG-49891 and ENSG00000144848 and 64422, ATG3 and IDBG-632300 and ENSBTAG00000009084 and 508571, Atg3 and IDBG-162821 and ENSMUSG00000022663 and 67841, Cytoplasm, enzyme binding, this GO :0000045 and autophagic vacuole assembly and biological process this GO :0000153 and cytoplasmic ubiquitin ligase complex and cellular component this GO :0000422 and mitochondrion degradation and biological process this GO :0005515 and protein binding and molecular function this GO :0005829 and cytosol and cellular component this GO :0006464 and cellular protein modification process and biological process this GO :0006612 and protein targeting to membrane and biological process this GO :0006914 and autophagy and biological process this GO :0016567 and protein ubiquitination and biological process this GO :0019776 and Atg8 ligase activity and molecular function this GO :0019777 and Atg12 ligase activity and molecular function this GO :0019787 and small conjugating protein ligase activity and molecular function this GO :0019899 and enzyme binding and molecular function this GO :0043653 and mitochondrial fragmentation involved in apoptotic process and biological process this GO :0044804 and nucleophagy and biological process this GO :1902017 and regulation of cilium assembly and biological process, this GO :0005515: protein binding, this GO :0005515: protein binding and also this GO :0019776: Atg8 ligase activity and also this GO :0019777: Atg12 ligase activity and also this GO :0019787: small conjugating protein ligase activity and also this GO :0019899: enzyme binding, this GO :0019776: Atg8 ligase activity, this GO :0019777: Atg12 ligase activity, this GO :0019787: small conjugating protein ligase activity, this GO :0019899: enzyme binding, autophagy related 3
Identity 20962
Gene ATG3
Long gene name autophagy related 3
Synonyms gene APG3L
Synonyms gene name APG3 autophagy 3-like (S, cerevisiae) , cerevisiae) ATG3 autophagy related 3 homolog (S
Locus 3q13, 2
Discovery year 2004-07-05
Entrez gene record 64422
Pubmed identfication 11825910
RefSeq identity NM_022488
Classification Autophagy related
Havana BLAST/BLAT OTTHUMG00000159260

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