| Antigen detectable with this ELISA kit | ATP Binding Cassette Transporter G1 (ABCG1) |
| Species reactivity | Homo sapiens (Human) |
| Range of detection | 0, 0, 0, 1, 2, 5ng/mL, 156-10ng/mL The standard curve concentrations used for the ELISA&rsquo, 156ng/mL, 25ng/mL, 313ng/mL, 5ng/mL, 625ng/mL, s were 10ng/mL |
| Sensitivity | 061ng/mL, The minimum detectable dose of this kit is typically less than 0 |
| Application | ELISA |
| Sample types tested for this ELISA kit | cell lysates and other biological fluids, Tissue homogenates |
| Time for performing t hourse test | 4, 5 hoursours |
| Test principle | 10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to ATP Binding Cassette Transporter G1 (ABCG1), The concentration of ATP Binding Cassette Transporter G1 (ABCG1) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to ATP Binding Cassette Transporter G1 (ABCG1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain ATP Binding Cassette Transporter G1 (ABCG1), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling | Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory | Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties | E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test | ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target | ATP Binding Cassette Transporter G1 (ABCG1) |
| Short name | ATP Binding Cassette Transporter G1 (ABCG1) ELISA kit |
| Technique | &hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host | antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name | member 1) Enzyme-linked immunosorbent assay reagent, sub-family G (WHITE), adenosine triphosphate Binding Cassette Transporter G1 (adenosine triphosphate-binding cassette |
| Alternative technique | elisas |
| Alternative to gene target | ABC8 and WHITE1, ABCG1 and IDBG-4231 and ENSG00000160179 and 9619, Abcg1 and IDBG-163644 and ENSMUSG00000024030 and 11307, BT, Cell surfaces, DNA-templated and biological process this GO :0006810 and transport and biological process this GO :0008203 and cholesterol metabolic process and biological process this GO :0009720 and detection of hormone stimulus and biological process this GO :0009897 and external side of plasma membrane and cellular component this GO :0010033 and response to organic substance and biological process this GO :0010745 and negative regulation of macrophage derived foam cell differentiation and biological process this GO :0010872 and regulation of cholesterol esterification and biological process this GO :0010875 and positive regulation of cholesterol efflux and biological process this GO :0010887 and negative regulation of cholesterol storage and biological process this GO :0010888 and negative regulation of lipid storage and biological process this GO :0015485 and cholesterol binding and molecular function this GO :0016020 and membrane and cellular component this GO :0016021 and integral component of membrane and cellular component this GO :0016887 and ATPase activity and molecular function this GO :0017127 and cholesterol transporter activity and molecular function this GO :0019534 and toxin transporter activity and molecular function this GO :0030301 and cholesterol transport and biological process this GO :0032367 and intracellular cholesterol transport and biological process this GO :0033344 and cholesterol efflux and biological process this GO :0033700 and phospholipid efflux and biological process this GO :0033993 and response to lipid and biological process this GO :0034041 and sterol-transporting ATPase activity and molecular function this GO :0034374 and low-density lipoprotein particle remodeling and biological process this GO :0034375 and high-density lipoprotein particle remodeling and biological process this GO :0034436 and glycoprotein transport and biological process this GO :0034437 and glycoprotein transporter activity and molecular function this GO :0042157 and lipoprotein metabolic process and biological process this GO :0042632 and cholesterol homeostasis and biological process this GO :0042803 and protein homodimerization activity and molecular function this GO :0042987 and amyloid precursor protein catabolic process and biological process this GO :0043531 and ADP binding and molecular function this GO :0043691 and reverse cholesterol transport and biological process this GO :0044281 and small molecule metabolic process and biological process this GO :0045087 and innate immune response and biological process this GO :0045542 and positive regulation of cholesterol biosynthetic process and biological process this GO :0046982 and protein heterodimerization activity and molecular function this GO :0046983 and protein dimerization activity and molecular function this GO :0055037 and recycling endosome and cellular component this GO :0055085 and transmembrane transport and biological process this GO :0055091 and phospholipid homeostasis and biological process this GO :0055099 and response to high density lipoprotein particle stimulus and biological process this GO :1901998 and toxin transport and biological process, member 1, protein dimerization activity, sub-family G (WHITE), this GO :0000139 and this GO lgi membrane and cellular component this GO :0003924 and GTPase activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005524 and ATP binding and molecular function this GO :0005525 and GTP binding and molecular function this GO :0005543 and phospholipid binding and molecular function this GO :0005548 and phospholipid transporter activity and molecular function this GO :0005739 and mitochondrion and cellular component this GO :0005768 and endosome and cellular component this GO :0005789 and endoplasmic reticulum membrane and cellular component this GO :0005794 and this GO lgi apparatus and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0005887 and integral component of plasma membrane and cellular component this GO :0006200 and ATP catabolic process and biological process this GO :0006355 and regulation of transcription, this GO :0003924: GTPase activity, this GO :0003924: GTPase activity and also this GO :0005515: protein binding and also this GO :0005524: ATP binding and also this GO :0005525: GTP binding and also this GO :0005543: phospholipid binding and also this GO :0005548: phospholipid transporter activity and also this GO :0015485: cholesterol binding and also this GO :0016887: ATPase activity and also this GO :0017127: cholesterol transporter activity and also this GO :0019534: toxin transporter activity and also this GO :0034041: sterol-transporting ATPase activity and also this GO :0034437: glycoprotein transporter activity and also this GO :0042803: protein homodimerization activity and also this GO :0043531: ADP binding and also this GO :0046982: protein heterodimerization activity and also this GO :0046983: protein dimerization activity, this GO :0005515: protein binding, this GO :0005524: ATP binding, this GO :0005525: GTP binding, this GO :0005543: phospholipid binding, this GO :0005548: phospholipid transporter activity, this GO :0015485: cholesterol binding, this GO :0016887: ATPase activity, this GO :0017127: cholesterol transporter activity, this GO :0019534: toxin transporter activity, this GO :0034041: sterol-transporting ATPase activity, this GO :0034437: glycoprotein transporter activity, this GO :0042803: protein homodimerization activity, this GO :0043531: ADP binding, this GO :0046982: protein heterodimerization activity, this GO :0046983: protein dimerization activity, 29337 and IDBG-639382 and ENSBTAG00000021272 and 510745, ATP-binding cassette |
| Identity | 73 |
| Gene | ABCG1 |
| Long gene name | ATP binding cassette subfamily G member 1 |
| Synonyms gene name | ATP-binding cassette, member 1 , sub-family G (WHITE) |
| Synonyms | ABC8 |
| Synonyms name | ATP-binding cassette transporter 8 |
| Locus | 21q22, 3 |
| Discovery year | 1999-10-26 |
| GenBank acession | U34919 |
| Entrez gene record | 9619 |
| Pubmed identfication | 8659545 16870176 |
| RefSeq identity | NM_207174 |
| Classification | ATP binding cassette subfamily G |
| Havana BLAST/BLAT | OTTHUMG00000086791 |