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ATP5E Antibody, FITC Conjugated

ATP5E Antibody, FITC Conjugated size: 0.1ml 333

Supplier BIOSS PRIMARY CONJUGATED ANTIBODIES 1
Price 333
Size 0.1ml
Contact us Ask Component cell markers and DNA bp. markers are genetic markerser genes or DNA sequenceswith selection audit
Long name FITC Conjugated, ATP5E Polyclonal Antibody
CategoryConjugated Primary Antibodies
ConjugationAnti-FITC Antibody
Host OrganismRabbit (Oryctolagus cuniculus)
Target AntigenATP5E
SpecificityThis is a highly specific antibody against ATP5E
ModificationUnmodified
Modification siteNone
ClonalityPolyclonal
ClonePolyclonal antibody
Concentration1ug per 1ul
SourceKLH conjugated synthetic peptide derived from human ATP5E
Gene ID number514
Tested applicationsIF(IHC-P)
Recommended dilutionsIF(IHC-P)(1:50-200)
CrossreactivityHuman
Crossreactive species details not every possible cross-reactivity is known, Due to limited amount of testing and knowledge
Antigen background ATPases contain two linked complexes: F1, F0 consists of three subunits: a, F1 consists of three Alpha chains and three Beta chains, Mitochondrial ATPase e chain (ATP5E) localizes to the mitochondria and catalyzes ATP synthesis, The e chain of F1 contains 50 amino acids and is the smallest of the five ATPase F1 chains, and F0, as well as one Gamma chain, b and c, one Delta chain and one e chain, the hydrophilic catalytic core, the membrane-embedded protein channel, which are weakly homologous, Mitochondrial ATP synthases (ATPases) transduce the energy contained in membrane electrochemical proton gradients into the energy required for synthesis of high-energy phosphate bonds
Purification methodThis antibody was purified via Protein A
Storage conditions 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA
Excitation Emission494nm/518nm
Properties Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°
Gene targetATP5E Conjugated
Short name FITC Conjugated, ATP5E Antibody
Technique Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody
LabelFITC
Alternative name fluorecein coupled, ATP5E (Antibody to)
Alternative techniqueantibodies
Identity 838
Gene ATP5E
Long gene name ATP synthase, H+ transporting, epsilon subunit , mitochondrial F1 complex
Locus 20q13, 32
Discovery year 1993-02-25
GenBank acession AF077045
Entrez gene record 514
Pubmed identfication 10727396
RefSeq identity NM_001001977
Classification F-type ATPases Mitochondrial complex V: ATP synthase subunits
Havana BLAST/BLAT OTTHUMG00000032854

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