| Category | Antibody |
| Concentration | 2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form | Antigen affinity purified |
| Conjugation | Unconjugated |
| Clone | Polyclonal antibody |
| Recognised antigen | B Raf (BRAF) |
| Host animal | Rabbit (Oryctolagus cuniculus) |
| Clonality | Polyclonal (rabbit origin) |
| Species reactivity | Due to limited knowledge and inability to test the antibody against all known species, Mouse (Mus musculus), Rat , we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications | WB |
| Recommended dilutions | 5-1ug/ml, Western blot: 0 |
| Notes | Titration of the B Raf antibody may be required due to differences in protocols and secondary/substrate sensitivity, The stated application concentrations are suggested starting points |
| Intented use | This B Raf antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot # | P15056 |
| Purity | Antigen affinity |
| Description | B Raf is a member of the Raf kinase family of serine/threonine-specific protein kinases, Northern blot analysis detected transcripts of 10 and 13 kb in cerebrum, Testis also showed lower expression of the 10- and 13-kb transcripts, This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, also known as RAFB1, and placenta and transcripts of 2, and secretion, differentiation, fetal brain, which affects cell division, 5 kb in testis, 6 and 4, BRAF (v-raf murine sarcoma viral oncogene homolog B1) is a human gene that makes a serine/threonine-protein kinase called B Raf |
| Immunogen | mouse and rat), Amino acids 344-362 (QPFRPADEDHRNQFGQRDR) were used as the immunogen for this B Raf antibody (100% homologous in human |
| Storage | Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, For long-term, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the B Raf antibody can be stored for up to one month at 4oC, After reconstitution |
| Properties | C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target | B Raf (BRAF) |
| Short name | B Raf Antibody (BRAF) |
| Technique | antibodies against human proteins, antibodies for, Antibody |
| Alternative name | B Raf (Antibody to) (v-raf murine sarcoma viral oncogene homolog B1) |
| Alternative technique | antibodies |
| Alternative to gene target | B-RAF1 and BRAF1 and NS7 and RAFB1, BRAF and IDBG-44351 and ENSG00000157764 and 673, BRAF and IDBG-636391 and ENSBTAG00000021761 and, Braf and IDBG-138657 and ENSMUSG00000002413 and 109880, alpha-beta T cell differentiation and biological process this GO :0043368 and positive T cell selection and biological process this GO :0043434 and response to peptide hormone and biological process this GO :0043524 and negative regulation of neuron apoptotic process and biological process this GO :0044297 and cell body and cellular component this GO :0046632 and alpha-beta T cell differentiation and biological process this GO :0046982 and protein heterodimerization activity and molecular function this GO :0048011 and neurotrophin TRK receptor signaling pathway and biological process this GO :0051291 and protein heterooli this GO merization and biological process this GO :0051496 and positive regulation of stress fiber assembly and biological process this GO :0051591 and response to cAMP and biological process this GO :0060291 and long-term synaptic potentiation and biological process this GO :0070374 and positive regulation of ERK1 and ERK2 cascade and biological process this GO :0070849 and response to epidermal growth factor and biological process this GO :0071277 and cellular response to calcium ion and biological process this GO :1900026 and positive regulation of substrate adhesion-dependent cell spreading and biological process this GO :2000301 and negative regulation of synaptic vesicle exocytosis and biological process this GO :2000352 and negative regulation of endothelial cell apoptotic process and biological process, nuclei, this GO :0000165 and MAPK cascade and biological process this GO :0000186 and activation of MAPKK activity and biological process this GO :0002318 and myeloid progenitor cell differentiation and biological process this GO :0004672 and protein kinase activity and molecular function this GO :0004674 and protein serine/threonine kinase activity and molecular function this GO :0004709 and MAP kinase kinase kinase activity and molecular function this GO :0004713 and protein tyrosine kinase activity and molecular function this GO :0005057 and receptor signaling protein activity and molecular function this GO :0005509 and calcium ion binding and molecular function this GO :0005515 and protein binding and molecular function this GO :0005524 and ATP binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0005739 and mitochondrion and cellular component this GO :0005829 and cytosol and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0006468 and protein phosphorylation and biological process this GO :0007165 and signal transduction and biological process this GO :0007264 and small GTPase mediated signal transduction and biological process this GO :0007268 and synaptic transmission and biological process this GO :0008542 and visual learning and biological process this GO :0008543 and fibroblast growth factor receptor signaling pathway and biological process this GO :0009887 and organ morphogenesis and biological process this GO :0010628 and positive regulation of gene expression and biological process this GO :0010764 and negative regulation of fibroblast migration and biological process this GO :0016020 and membrane and cellular component this GO :0016772 and transferase activity, this GO :0004672: protein kinase activity, this GO :0004672: protein kinase activity and also this GO :0004674: protein serine/threonine kinase activity and also this GO :0004709: MAP kinase kinase kinase activity and also this GO :0004713: protein tyrosine kinase activity and also this GO :0005057: receptor signaling protein activity and also this GO :0005509: calcium ion binding and also this GO :0005515: protein binding and also this GO :0005524: ATP binding and also this GO :0016772: transferase activity, this GO :0004674: protein serine/threonine kinase activity, this GO :0004709: MAP kinase kinase kinase activity, this GO :0004713: protein tyrosine kinase activity, this GO :0005057: receptor signaling protein activity, this GO :0005509: calcium ion binding, this GO :0005515: protein binding, this GO :0005524: ATP binding, this GO :0016772: transferase activity, this GO :0031434: mitogen-activated protein kinase kinase binding, this GO :0042802: identical protein binding, this GO :0046982: protein heterodimerization activity, transferase activity, transferring phosphorus-containing groups, transferring phosphorus-containing groups and also this GO :0031434: mitogen-activated protein kinase kinase binding and also this GO :0042802: identical protein binding and also this GO :0046982: protein heterodimerization activity, transferring phosphorus-containing groups and molecular function this GO :0030154 and cell differentiation and biological process this GO :0031434 and mitogen-activated protein kinase kinase binding and molecular function this GO :0033138 and positive regulation of peptidyl-serine phosphorylation and biological process this GO :0035019 and somatic stem cell maintenance and biological process this GO :0035556 and intracellular signal transduction and biological process this GO :0035690 and cellular response to drug and biological process this GO :0042127 and regulation of cell proliferation and biological process this GO :0042802 and identical protein binding and molecular function this GO :0043005 and neuron projection and cellular component this GO :0043066 and negative regulation of apoptotic process and biological process this GO :0043367 and CD4-positive, v-raf murine sarcoma viral oncogene homolog B1 |
| Identity | 1097 |
| Gene | BRAF |
| Long gene name | B-Raf proto-oncogene, serine/threonine kinase |
| Synonyms gene name | v-raf murine sarcoma viral oncogene homolog B |
| Synonyms | BRAF1 |
| Locus | 7q34 |
| Discovery year | 1991-07-16 |
| GenBank acession | M95712 |
| Entrez gene record | 673 |
| Pubmed identfication | 2284096 1565476 |
| RefSeq identity | NM_004333 |
| Classification | RAF family Mitogen-activated protein kinase kinase kinases |
| Havana BLAST/BLAT | OTTHUMG00000157457 |
| Locus Specific Databases | LRG_299 |
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