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B-Raf (Ser365) Antibody, Biotin Conjugated

B-Raf (Ser365) Antibody, Biotin Conjugated size: 0.1ml 350

Price 350
Size 0.1ml
Long name Biotin Conjugated, B-Raf (Ser365) Polyclonal Antibody
CategoryConjugated Primary Antibodies
ConjugationBiotinylated
Host OrganismRabbit (Oryctolagus cuniculus)
Target AntigenB-Raf Ser365
Specificity This phophorylation site is homologous to that of Ser348 in Mouse and Ser347 in Rat, This is a highly specific antibody against B-Raf Ser365
ModificationPhosphorylation
Modification siteSer365
ClonalityPolyclonal
ClonePolyclonal antibody
Concentration1ug per 1ul
Immunogen_range350-390/766
Subcellular location Cell membrane, Nucleus, Cytoplasm
SourceKLH conjugated synthetic phosphopeptide derived from human B Raf around the phosphorylation site of Ser365
Gene ID number673
Swiss ProtP15056
Tested applications IHC-P, WB
Recommended dilutions IHC-P(1:100-500), WB(1:100-1000)
Crossreactivity Mouse, Rat, Human
Crossreactive species details not every possible cross-reactivity is known, Due to limited amount of testing and knowledge
Antigen background May play a role in the postsynaptic responses of hippocampal neuron, Phosphorylates MAP2K1, and thereby contributes to the MAP kinase signal transduction pathway, Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus
Purification methodThis antibody was purified via Protein A
Storage conditions 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA
Excitation EmissionN/A
Synonyms B-RAF1, BRAF, BRAF1, Proto-oncogene B-Raf, RAFB1, Serine/threonine-protein kinase B-raf, p94, v-Raf murine sarcoma viral oncogene homolog B1, NS7
Properties Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, Biotin conjugates can be detected by horseradish peroxidase, To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, alkaline phosphatase substrates or anti biotin conjugated antibodies, 6, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°
Gene targetB-Raf (Ser365) Conjugated
Short name Conjugated, B-Raf (Ser365) Antibody
Technique antibodies against human proteins, antibodies for, antibody Conjugates, Antibody
LabelBiotin
Alternative name biotinilated coupled, B-Raf (Ser365) (Antibody to)
Alternative techniqueantibodies

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