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B-Raf (Thr401) Antibody, FITC Conjugated

B-Raf (Thr401) Antibody, FITC Conjugated size: 0.1ml 350

Supplier BIOSS PRIMARY CONJUGATED ANTIBODIES 1
Price 350
Size 0.1ml
Contact us Ask Component cell markers and DNA bp. markers are genetic markerser genes or DNA sequenceswith selection audit
Long name FITC Conjugated, B-Raf (Thr401) Polyclonal Antibody
CategoryConjugated Primary Antibodies
ConjugationAnti-FITC Antibody
Host OrganismRabbit (Oryctolagus cuniculus)
Target AntigenB-Raf Thr401
SpecificityThis is a highly specific antibody against B-Raf Thr401
ModificationPhosphorylation
Modification siteThr401
ClonalityPolyclonal
ClonePolyclonal antibody
Concentration1ug per 1ul
SourceKLH conjugated synthetic phosphopeptide derived from human B-Raf around the phosphorylation site of Thr401
Gene ID number673
Tested applicationsIF(IHC-P)
Recommended dilutionsIF(IHC-P)(1:50-200)
Crossreactivity Mouse, Rat, Human
Crossreactive species details not every possible cross-reactivity is known, Due to limited amount of testing and knowledge
Antigen background 1 BRAF is important for the regulation of cell proliferation and determination of cell fate during embryogenesis, B-Raf is a serine/threonine protein kinase that acts as a signal transducer from membrane-associated receptors to nuclear transcription factors, BRAF acts downstream of Ras and upstream of MEK in the Ras-Raf-MEK-ERK signal transduction pathway, Defects in BRAF are involved in a wide range of cancers, Interaction between Ras proteins and Raf proteins results in Raf-mediated phosphorylation and activation of MEK (also known as MAP kinase kinase), Raf protein family members, and differentiation in response to growth factors, and hormones, cytokines, have intrinsic serine/threonine kinase activity, including A Raf and B Raf, proliferation, which is a conserved RAS-activated protein kinase cascade that regulates cell growth, The Raf kinases are important intermediates in signal transduction
Purification methodThis antibody was purified via Protein A
Storage conditions 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA
Excitation Emission494nm/518nm
Synonyms 94 kDa B raf protein, B Raf proto oncogene serine threonine protein kinase, B raf 1, BRAF 1, BRAF1, BRAF_HUMAN, Braf, FLJ95109, MGC126806, MGC138284, Murine sarcoma viral v raf oncogene homolog B1, Murine sarcoma viral v-raf oncogene homolog B1, RAFB 1, RAFB1, cRmil, p-B Raf phospho T401, p94, v raf murine sarcoma viral oncogene homolog B1, B Raf phospho T401
Properties Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°
Gene targetB-Raf (Thr401) Conjugated
Short name FITC Conjugated, B-Raf (Thr401) Antibody
Technique Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody
LabelFITC
Alternative name fluorecein coupled, B-Raf (Thr401) (Antibody to)
Alternative techniqueantibodies

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