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BHLHB5 Antibody, Biotin Conjugated

BHLHB5 Antibody, Biotin Conjugated size: 0.1ml 333

Price 333
Size 0.1ml
Long name Biotin Conjugated, BHLHB5 Polyclonal Antibody
CategoryConjugated Primary Antibodies
ConjugationBiotinylated
Host OrganismRabbit (Oryctolagus cuniculus)
Target AntigenBHLHB5
SpecificityThis is a highly specific antibody against BHLHB5
ModificationUnmodified
Modification siteNone
ClonalityPolyclonal
ClonePolyclonal antibody
Concentration1ug per 1ul
Immunogen_range255-305/381
Subcellular locationNucleus
SourceKLH conjugated synthetic peptide derived from human BHLHB5
Gene ID number27319
Tested applications IHC-P, WB
Recommended dilutions IHC-P(1:100-500), WB(1:100-1000)
Crossreactivity Mouse, Rat, Human
Crossreactive species details not every possible cross-reactivity is known, Due to limited amount of testing and knowledge
Antigen background BETA 3 contains a putative DNA-binding domain, BETA 3 is a protein that is functionally similar to members of the Id family in that it can inhibit the binding of E47 homodimers as well as E47/Neuro D and E47/Myo D heterodimers to consensus DNA sequences , Class A proteins heterodimerize with class B proteins to activate transcription, Class A proteins include the ubiquitously expressed E-box binding factors E12/E47, Class B proteins such as Myo D, For instance, ITF2 and HEB (BETA 1 or HTF4), In contrast to members of the Id family, The Id family can therefore form heterodimers with the myogenic family, Working in opposition to these positively acting factors are a specialized group of proteins that function as dominant negative regulators, but the resulting complexes are transcriptionally inactive, myogenin and Neuro D (BETA 2) are transiently expressed and exhibit a more limited tissue distribution, the Id family of transcriptional repressors contains a HLH region required for dimerization but lacks a functional DNA-binding domain, Members of the myogenic determination family are basic helix-loop-helix (bHLH) proteins that can be separated into two classes
Purification methodThis antibody was purified via Protein A
Storage conditions 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA
Excitation EmissionN/A
Synonyms CAGL85 Beta3 bHLHe22
Properties Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, Biotin conjugates can be detected by horseradish peroxidase, To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, alkaline phosphatase substrates or anti biotin conjugated antibodies, 6, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°
Gene targetBHLHB5 Conjugated
Short name Conjugated, BHLHB5 Antibody
Technique antibodies against human proteins, antibodies for, antibody Conjugates, Antibody
LabelBiotin
Alternative name biotinilated coupled, BHLHB5 (Antibody to)
Alternative techniqueantibodies
Identity 11963
Gene BHLHE22
Long gene name basic helix-loop-helix family member e22
Synonyms gene TNRC20 BHLHB5
Synonyms gene name 5 basic helix-loop-helix family, class B, member e22 , trinucleotide repeat containing 20 basic helix-loop-helix domain containing
Locus 8q12, 3
Discovery year 2000-01-27
GenBank acession U80755
Entrez gene record 27319
Pubmed identfication 9225980 12213201 18557763
RefSeq identity NM_152414
Classification Basic helix-loop-helix proteins Trinucleotide repeat containing
Havana BLAST/BLAT OTTHUMG00000164386

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