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Bid (Ser65) Antibody, Biotin Conjugated

Bid (Ser65) Antibody, Biotin Conjugated size: 0.1ml 350

Supplier BIOSS PRIMARY CONJUGATED ANTIBODIES 1
Price 350
Size 0.1ml
Contact us Ask Component cell markers and DNA bp. markers are genetic markerser genes or DNA sequenceswith selection audit
Long name Biotin Conjugated, Bid (Ser65) Polyclonal Antibody
CategoryConjugated Primary Antibodies
ConjugationBiotinylated
Host OrganismRabbit (Oryctolagus cuniculus)
Target AntigenBid Ser65
SpecificityThis is a highly specific antibody against Bid Ser65
ModificationPhosphorylation
Modification siteSer65
ClonalityPolyclonal
ClonePolyclonal antibody
Concentration1ug per 1ul
SourceKLH conjugated synthetic phosphopeptide derived from rat Bid around the phosphorylation site of Ser65
Gene ID number64625
Tested applications IHC-P, WB
Recommended dilutions IHC-P(1:100-500), WB(1:100-1000)
CrossreactivityRat
Crossreactive species details not every possible cross-reactivity is known, Due to limited amount of testing and knowledge
Antigen background Bid deficient mice are found resistant to the lethal effects of death factor signals relayed through Fas, Cleaved Bid translocates to mitochondria and releases its potent proapoptotic activity, In animal model studies, Its active form is generated upon proteolytic cleavage by caspase 8 in the Fas signaling pathway, Mutation in the SH3 domain can diminish the cytochrome c releasing activity, The cytochrome c releasing activity of Bid was antagonized by Bcl2, a BH3 domain containing proapoptotic Bcl2 family member, is localized in the cytosolic fraction of cells as an inactive precursor, which in turn induces cytochrome c release and mitochondrial damage, Bid
Purification methodThis antibody was purified via Protein A
Storage conditions 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA
Excitation EmissionN/A
Synonyms AltName: BH3-interacting domain death agonist p15, BH3 interacting domain death agonist, BH3-interacting domain death agonist, BH3-interacting domain death agonist p11, BH3-interacting domain death agonist p13, BID, p-Bid phospho S65, p11 BID, p13 BID, p22 BID, Bid phospho S65
Properties Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, Biotin conjugates can be detected by horseradish peroxidase, To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, alkaline phosphatase substrates or anti biotin conjugated antibodies, 6, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°
Gene targetBid (Ser65) Conjugated
Short name Conjugated, Bid (Ser65) Antibody
Technique antibodies against human proteins, antibodies for, antibody Conjugates, Antibody
LabelBiotin
Alternative name biotinilated coupled, BH3 interacting domain death a this GO nist (Ser65) (Antibody to)
Alternative techniqueantibodies

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