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BIG1/ARFGEF1 Antibody, Biotin Conjugated

BIG1/ARFGEF1 Antibody, Biotin Conjugated size: 0.1ml 333

Supplier BIOSS PRIMARY CONJUGATED ANTIBODIES 1
Price 333
Size 0.1ml
Contact us Ask Component cell markers and DNA bp. markers are genetic markerser genes or DNA sequenceswith selection audit
Long name Biotin Conjugated, BIG1/ARFGEF1 Polyclonal Antibody
CategoryConjugated Primary Antibodies
ConjugationBiotinylated
Host OrganismRabbit (Oryctolagus cuniculus)
Target AntigenBIG1/ARFGEF1
SpecificityThis is a highly specific antibody against BIG1/ARFGEF1
ModificationUnmodified
Modification siteNone
ClonalityPolyclonal
ClonePolyclonal antibody
Concentration1ug per 1ul
SourceKLH conjugated synthetic peptide derived from human BIG1/ARFGEF1
Gene ID number10565
Tested applications IHC-P, WB
Recommended dilutions IHC-P(1:100-500), WB(1:100-1000)
Crossreactivity Mouse, Rat, Human
Crossreactive species details not every possible cross-reactivity is known, Due to limited amount of testing and knowledge
Antigen background BIG1 and BIG2 exist in macromolecular complexes that move between the Golgi membranes and cytosol, BIG2 associates with PKA regulatory subunits, Both GEPS contain a sec7 domain that is responsible for their brefeldin inhibition and also their catalytic activity, GEPs comprise two major families, In vivo, Two genes for GEPs found on human chromosomes 8 and 20 encode BFA sensitive GEPs designated BIG1 and BIG2, a family of guanine nucleotide-binding proteins that play an important role in intracellular vesicular trafficking, a protein that effects Golgi structure and a group of smaller GEPs that are insenstive to BFA, implying that BIG2 may act as an A kinase-anchoring protein (AKAP) that could coordinate the cAMP and ARF regulatory pathways, large GEPs that are inhibited by brefeldin A (BFA), Guanine nucleotide-exchange proteins (GEPs) accelerate replacement of bound GDP with GTP and thereby activate ADP-ribosylation factors (ARFs)
Purification methodThis antibody was purified via Protein A
Storage conditions 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA
Excitation EmissionN/A
Synonyms DKFZP434L057 BIG1 ARFGEP1 p200
Properties Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, Biotin conjugates can be detected by horseradish peroxidase, To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, alkaline phosphatase substrates or anti biotin conjugated antibodies, 6, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°
Gene targetBIG1/ARFGEF1 Conjugated
Short name Conjugated, BIG1/ARFGEF1 Antibody
Technique antibodies against human proteins, antibodies for, antibody Conjugates, Antibody
LabelBiotin
Alternative name biotinilated coupled, BIG1/ADP-ribosylation factor guanine nucleotide-exchange factor 1 (brefeldin A-inhibited) (Antibody to)
Alternative techniqueantibodies
Alternative to gene target ARFGEF1 and IDBG-24344 and ENSG00000066777 and 10565, ARFGEF1 and IDBG-644666 and ENSBTAG00000014691 and 281022, ARFGEP1 and BIG1 and P200, Arfgef1 and IDBG-133303 and ENSMUSG00000067851 and 211673, nuclei, protein kinase A regulatory subunit binding, this GO :0000139 and this GO lgi membrane and cellular component this GO :0005085 and guanyl-nucleotide exchange factor activity and molecular function this GO :0005086 and ARF guanyl-nucleotide exchange factor activity and molecular function this GO :0005488 and binding and molecular function this GO :0005515 and protein binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005730 and nucleolus and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0005794 and this GO lgi apparatus and cellular component this GO :0005802 and trans- this GO lgi network and cellular component this GO :0005829 and cytosol and cellular component this GO :0006887 and exocytosis and biological process this GO :0007030 and this GO lgi organization and biological process this GO :0010256 and endomembrane system organization and biological process this GO :0015031 and protein transport and biological process this GO :0016192 and vesicle-mediated transport and biological process this GO :0016363 and nuclear matrix and cellular component this GO :0017022 and myosin binding and molecular function this GO :0030532 and small nuclear ribonucleoprotein complex and cellular component this GO :0030837 and negative regulation of actin filament polymerization and biological process this GO :0032012 and regulation of ARF protein signal transduction and biological process this GO :0034237 and protein kinase A regulatory subunit binding and molecular function this GO :0034259 and negative regulation of Rho GTPase activity and biological process this GO :0043547 and positive regulation of GTPase activity and biological process this GO :0048471 and perinuclear region of cytoplasm and cellular component this GO :0090284 and positive regulation of protein glycosylation in this GO lgi and biological process this GO :0090303 and positive regulation of wound healing and biological process this GO :2000114 and regulation of establishment of cell polarity and biological process, this GO :0005085: guanyl-nucleotide exchange factor activity, this GO :0005085: guanyl-nucleotide exchange factor activity and also this GO :0005086: ARF guanyl-nucleotide exchange factor activity and also this GO :0005488: binding and also this GO :0005515: protein binding and also this GO :0017022: myosin binding and also this GO :0034237: protein kinase A regulatory subunit binding, this GO :0005086: ARF guanyl-nucleotide exchange factor activity, this GO :0005488: binding, this GO :0005515: protein binding, this GO :0017022: myosin binding, this GO :0034237: protein kinase A regulatory subunit binding, ADP-ribosylation factor guanine nucleotide-exchange factor 1 (brefeldin A-inhibited)
Identity 15772
Gene ARFGEF1
Long gene name ADP ribosylation factor guanine nucleotide exchange factor 1
Synonyms gene name ADP-ribosylation factor guanine nucleotide-exchange factor 1 (brefeldin A-inhibited)
Synonyms name Brefeldin A-inhibited guanine nucleotide-exchange protein 1
Locus 8q13, 2
Discovery year 2004-08-09
GenBank acession AF084520
Entrez gene record 10565
Pubmed identfication 10212200 8917509
RefSeq identity NM_006421
Havana BLAST/BLAT OTTHUMG00000164626

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