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BrdU Antibody, Biotin Conjugated

BrdU Antibody, Biotin Conjugated size: 0.1ml 333

Price 333
Size 0.1ml
Long name Biotin Conjugated, BrdU Polyclonal Antibody
CategoryConjugated Primary Antibodies
ConjugationBiotinylated
Host OrganismRabbit (Oryctolagus cuniculus)
Target AntigenBrdU
SpecificityThis is a highly specific antibody against BrdU
ModificationUnmodified
Modification siteNone
ClonalityPolyclonal
ClonePolyclonal antibody
Concentration1ug per 1ul
SourceBrdU conjugated to KLH
Tested applicationsIHC-P
Recommended dilutionsIHC-P(1:100-500)
CrossreactivityOthers
Crossreactive species detailsBrdU
Antigen background Also dynamic proliferative information such as the S-phase transit rate and the potential doubling time can be obtained, In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal is an accurate and comprehensive method to quantitate the degree of DNA-synthesis, by means of bivariate BrdU/DNA flow cytometric analysis, BrdU is incorporated into the newly synthezised DNA of S-phase cells may provide an estimate for the fraction of cells in S-phase, The immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells
Purification methodThis antibody was purified via Protein A
Storage conditions 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA
Excitation EmissionN/A
Synonyms 5-BrdU, 5-Bromo-2-deoxyuridine, Bromodeoxyuridine, proliferation Marker, Bromodeoxyuridine
Properties Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, Biotin conjugates can be detected by horseradish peroxidase, To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, alkaline phosphatase substrates or anti biotin conjugated antibodies, 6, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°
Gene targetBrdU Conjugated
Short name Conjugated, BrdU Antibody
Technique antibodies against human proteins, antibodies for, antibody Conjugates, Antibody
LabelBiotin
Alternative name biotinilated coupled, Bromodeoxyuridine (Antibody to)
Alternative techniqueantibodies

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