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c-Fos (Thr325) Antibody, Biotin Conjugated

c-Fos (Thr325) Antibody, Biotin Conjugated size: 0.1ml 350

Supplier BIOSS PRIMARY CONJUGATED ANTIBODIES 1
Price 350
Size 0.1ml
Contact us Ask Component cell markers and DNA bp. markers are genetic markerser genes or DNA sequenceswith selection audit
Long name Biotin Conjugated, c-Fos (Thr325) Polyclonal Antibody
CategoryConjugated Primary Antibodies
ConjugationBiotinylated
Host OrganismRabbit (Oryctolagus cuniculus)
Target Antigenc-Fos Thr325
Specificity This phosphorylation site is homologous to Thr325 in Mouse and Rat, This is a highly specific antibody against c-Fos Thr325
ModificationPhosphorylation
Modification siteThr325
ClonalityPolyclonal
ClonePolyclonal antibody
Concentration1ug per 1ul
Immunogen_range300-350/380
Subcellular location Nucleus, Cytoplasm
SourceKLH conjugated synthetic phosphopeptide derived from human c-Fos around the phosphorylation site of Thr325
Gene ID number2353
Swiss ProtP01100
Tested applications IHC-P, WB
Recommended dilutions IHC-P(1:100-500), WB(1:100-1000)
Crossreactivity Mouse, Rat, Human
Crossreactive species details not every possible cross-reactivity is known, Due to limited amount of testing and knowledge
Antigen background FOS and JUN/AP-1 basic regions each seems to interact with symmetrical DNA half sites, Has a critical function in regulating the development of cells destined to form and maintain the skeleton, In growing cells, In the heterodimer, It is thought to have an important role in signal transduction, On TGF-beta activation, This activity requires Tyr-dephosphorylation and association with the endoplasmic reticulum, activates phospholipid synthesis, cell proliferation and differentiation, forms a multimeric SMAD3/SMAD4/JUN/FOS complex at the AP1/SMAD-binding site to regulate TGF-beta-mediated signaling, possibly by activating CDS1 and PI4K2A, Nuclear phosphoprotein which forms a tight but non-covalently linked complex with the JUN/AP-1 transcription factor
Purification methodThis antibody was purified via Protein A
Storage conditions 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA
Excitation EmissionN/A
Synonyms AP-1, C-FOS, Cellular oncogene fos, FOS, G0/G1 switch regulatory protein 7, G0S7, Proto-oncogene c-Fos, p55
Properties Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, Biotin conjugates can be detected by horseradish peroxidase, To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, alkaline phosphatase substrates or anti biotin conjugated antibodies, 6, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°
Gene targetc-Fos (Thr325) Conjugated
Short name Conjugated, c-Fos (Thr325) Antibody
Technique antibodies against human proteins, antibodies for, antibody Conjugates, Antibody
LabelBiotin
Alternative name biotinilated coupled, c-FBJ murine osteosarcoma viral oncogene homolog (Thr325) (Antibody to)
Alternative techniqueantibodies

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