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Calpain 2 Antibody, FITC Conjugated

Calpain 2 Antibody, FITC Conjugated size: 0.1ml 333

Price 333
Size 0.1ml
Long name FITC Conjugated, Calpain 2 Polyclonal Antibody
CategoryConjugated Primary Antibodies
ConjugationAnti-FITC Antibody
Host OrganismRabbit (Oryctolagus cuniculus)
Target AntigenCalpain 2
SpecificityThis is a highly specific antibody against Calpain 2
ModificationUnmodified
Modification siteNone
ClonalityPolyclonal
ClonePolyclonal antibody
Concentration1ug per 1ul
SourceKLH conjugated synthetic peptide derived from mouse Calpain 2
Gene ID number824
Tested applicationsIF(IHC-P)
Recommended dilutionsIF(IHC-P)(1:50-200)
Crossreactivity Mouse, Rat, Human
Crossreactive species details not every possible cross-reactivity is known, Due to limited amount of testing and knowledge
Antigen background Both Calpain 1 and Calpain 2 are ubiquitously expressed, Both Calpains 1 and 2 are composed of an 80 kD subunit and a 30 kD subunit, Domains in the large subunit include the amino terminal domain I, It is not clear that all calpains contain a small subunit, Mu calpain has a micromolar sensitivity (thus the mu) as compared to the millimolar calcium sensitivity of m calpain, Other calpain family members (calpain 94, The calpain family members consist of a common small subunit (Calpain 4), The calpains appear to serve multiple physiological roles, The calpains have papain like activity, Whereas the 30 kDa subunit is shared by both enzymes, also known as Calpain 2, and a large variable subunit, and are countered by the endogenous calpain inhibitor, and ideas concerning the functions of these enzymes are in a state of rapid flux, and m calpain, and perhaps different functions, and the EF hand domain IV, are intracellular, calcium dependent cysteine proteases, calpastatin, domain III, etc) have more limited tissue distribution, ncl2, ncl3, the larger catalytic subunits are different and exhibit the distinct Ca++ requirements that are suggested by their names, the proteinase domain II, thus the pain nomenclature, Calpain
Purification methodThis antibody was purified via Protein A
Storage conditions 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA
Excitation Emission494nm/518nm
Synonyms CALP80, CALP80, CAN2_MOUSE, CANP 2, CANP L2, CANP2, CANPL 2, CANPL2, CANPml, CAPN 2, CAPN2, Calcium activated neutral proteinase, Calcium-activated neutral proteinase 2, Calpain 2, Calpain 2 catalytic subunit, Calpain 2 large catalytic subunit, Calpain 2 large subunit, Calpain 2 m/II large subunit, Calpain M type, Calpain M-type, Calpain large polypeptide L2, Calpain-2 catalytic subunit, Calpain-2 large subunit, Calpain2, Capa2, FLJ39928, M calpain, M calpin, M type, M-calpain, Millimolar calpain, Millimolar-calpain, large [catalytic] subunit, mCANP, Calcium activated neutral proteinase 2
Properties Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°
Gene targetCalpain 2 Conjugated
Short name FITC Conjugated, Calpain 2 Antibody
Technique Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody
LabelFITC
Alternative name fluorecein coupled, Calpain 2 (Antibody to)
Alternative techniqueantibodies

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