| Alternative name 1 | CD40 Ligand, Clone: 39H5, FITC, Hamster Mab anti-Mouse, flow, CD154 |
| Alternative name 2 | CD40 Ligand, Clone: 39H5, FITC, Hamster Mab antibody-Mouse, flow, CD154 |
| Alternative name 3 | CD40 Ligand, Clone: 39H5, FITC, Hamster Mab antibody-Mus musculus, flow, CD154 |
| Alternative name 4 | anti-CD154 CD40 Ligand Clone: 39H5 flow |
| Alternative name 5 | CD154 CD40 Ligand Clone: 39H5 flow |
| Clonality | Monoclonal Antibody |
| Description | Receptor agonists are often tested for drug development, FAS ligand and other ligands are binding to the receptor for signaling pathways for example in apoptosis or JNK signaling |
| Storage and handling | Antibodies in a liquid form can be shipped and stored for a short period of time at +4 degrees Celsius, antibodies that are lyophilized can be transported at ambient temperature and stored for short terms at +4 degrees Celsius, for long term storage (up to one year) 25-50% glycerol or ethylene glycol should be added and then the container has to be stored at -20°, for longer periods - at -20 , C, Generally |
| Advisory | For antibodies in a liquid form or reconstituted lyophilized antibodies small amounts of the soultion could be captured on the cap or the walls of the container, Right before use you could briefly centrifuge the vial to collect all of the solution on the bottom, To keep the quality and the affinity of the antibody cycles of freezing and thawing should be avoided |
| Properties | Armenian of Syrian hamster monoclonal IgGs are useful antibodies for double staining your IHC samples, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, Using mabs or monoclonal antibodies has a lot of advantages for Flow cytometry, When excited at 488 nanometers, accurate-monoclonals FITC is highly sensitive to pH extremes, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, This accurate-monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS |
| Conjugation | Anti-FITC Antibody |
| About | Each murine monoclonal anibody has his own affinity specific for the clone, Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes, Monoclonals of this antigen are available in different clones |
| Test | A/J, BALB/c, Mouse are mature after 40 days for females and 55 days for males, SCID while the CD-1 is outbred as strain, The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year, Transgenic, congenic and inbread strains are known for C57BL/6, knock-out, Mouse , mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab, or |
| Latin name | Mus musculus |
| French translation | anticorps |
| Gene target | 39H5, CD40 Ligand, Monoclonal, CD154 |
| Short name | CD40 Ligand, Clone: 39H5, FITC, Mouse Monoclonal antibody-Mouse, CD154 |
| Technique | Fluorescein, Monoclonals or monoclonal antibodies, mouses, Mouse |
| Host | Hamster |
| Label | FITC |
| Species | Hamsters, Mouses, Mouse |
| Alternative name | CD40 molecule, Hamster Mouse monoclonal (Antibody to)-Mouse, clonality: 39H5, flow, fluorecein, tumor necrosis factor receptor superfamily member 5 Ligand, CD154 |
| Alternative technique | murine |
| Alternative to gene target | BT, Bp50 and CDW40 and p50 and TNFRSF5, CD40 and IDBG-78904 and ENSG00000101017 and 958, Cd40 and IDBG-212533 and ENSMUSG00000017652 and 21939, Extracellular, TNF receptor superfamily member 5, this GO :0001934 and positive regulation of protein phosphorylation and biological process this GO :0002768 and immune response-regulating cell surface receptor signaling pathway and biological process this GO :0003823 and antigen binding and molecular function this GO :0004871 and signal transducer activity and molecular function this GO :0004872 and receptor activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005615 and extracellular space and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0005887 and integral component of plasma membrane and cellular component this GO :0006461 and protein complex assembly and biological process this GO :0006874 and cellular calcium ion homeostasis and biological process this GO :0006954 and inflammatory response and biological process this GO :0009897 and external side of plasma membrane and cellular component this GO :0009986 and cell surface and cellular component this GO :0019899 and enzyme binding and molecular function this GO :0030168 and platelet activation and biological process this GO :0030890 and positive regulation of B cell proliferation and biological process this GO :0031625 and ubiquitin protein ligase binding and molecular function this GO :0032735 and positive regulation of interleukin-12 production and biological process this GO :0032855 and positive regulation of Rac GTPase activity and biological process this GO :0035631 and CD40 receptor complex and cellular component this GO :0042100 and B cell proliferation and biological process this GO :0042113 and B cell activation and biological process this GO :0042511 and positive regulation of tyrosine phosphorylation of Stat1 protein and biological process this GO :0043089 and positive regulation of Cdc42 GTPase activity and biological process this GO :0043123 and positive regulation of I-kappaB kinase/NF-kappaB signaling and biological process this GO :0043231 and intracellular membrane-bounded organelle and cellular component this GO :0043406 and positive regulation of MAP kinase activity and biological process this GO :0045087 and innate immune response and biological process this GO :0045944 and positive regulation of transcription from RNA polymerase II promoter and biological process this GO :0048304 and positive regulation of isotype switching to IgG isotypes and biological process this GO :0050776 and regulation of immune response and biological process this GO :0051023 and regulation of immunoglobulin secretion and biological process this GO :0051092 and positive regulation of NF-kappaB transcription factor activity and biological process this GO :0051607 and defense response to virus and biological process this GO :0070062 and extracellular vesicular exosome and cellular component this GO :0071222 and cellular response to lipopolysaccharide and biological process this GO :0071260 and cellular response to mechanical stimulus and biological process this GO :0090037 and positive regulation of protein kinase C signaling and biological process this GO :2000353 and positive regulation of endothelial cell apoptotic process and biological process, this GO :0003823: antigen binding, this GO :0003823: antigen binding and also this GO :0004871: signal transducer activity and also this GO :0004872: receptor activity and also this GO :0005515: protein binding and also this GO :0019899: enzyme binding and also this GO :0031625: ubiquitin protein ligase binding, this GO :0004871: signal transducer activity, this GO :0004872: receptor activity, this GO :0005515: protein binding, this GO :0019899: enzyme binding, this GO :0031625: ubiquitin protein ligase binding, ubiquitin protein ligase binding, 42498 and IDBG-643036 and ENSBTAG00000020736 and 286849, CD40 molecule |
| Identity | 11935 |
| Gene | CD40LG |
| Long gene name | CD40 ligand |
| Synonyms gene | HIGM1 IMD3 TNFSF5 |
| Synonyms gene name | member 5 (hyper-IgM syndrome) , tumor necrosis factor (ligand) superfamily |
| Synonyms | CD40L TRAP gp39 hCD40L CD154 |
| Synonyms name | CD40 antigen ligand tumor necrosis factor (ligand) superfamily member 5 T-B cell-activating molecule TNF-related activation protein hyper-IgM syndrome |
| Locus | Xq26, 3 |
| Discovery year | 1989-06-30 |
| GenBank acession | X67878 |
| Entrez gene record | 959 |
| Pubmed identfication | 1427881 7678782 |
| RefSeq identity | NM_000074 |
| Classification | Endogenous ligands Tumor necrosis factor superfamily CD molecules |
| Havana BLAST/BLAT | OTTHUMG00000022512 |
| Locus Specific Databases | CD40Lbase: Mutation registry for X-linked Hyper-IgM syndrome Mental Retardation database LRG_141 |
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