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CD20 Antibody, FITC Conjugated

CD20 Antibody, FITC Conjugated size: 0.1ml 333

Price 333
Size 0.1ml
Long name FITC Conjugated, CD20 Polyclonal Antibody
CategoryConjugated Primary Antibodies
ConjugationAnti-FITC Antibody
Host OrganismRabbit (Oryctolagus cuniculus)
Target AntigenCD20
SpecificityThis is a highly specific antibody against CD20
ModificationUnmodified
Modification siteNone
ClonalityPolyclonal
ClonePolyclonal antibody
Concentration1ug per 1ul
Immunogen_range240-290/297
Subcellular locationCytoplasm
SourceKLH conjugated synthetic peptide derived from human CD20
Gene ID number931
Swiss ProtP11836
Tested applicationsIF(IHC-P)
Recommended dilutionsIF(IHC-P)(1:50-200)
Crossreactivity Mouse, Rat, Human
Crossreactive species details not every possible cross-reactivity is known, Due to limited amount of testing and knowledge
Antigen background Although not a member of the tetraspanin superfamily of cell surface receptors, Low level expression of the CD20 antigen has been detected on normal T lymphocytes, The CD20 antigen is present on human pre B lymphocytes and on B lymphocytes at all stages of maturation, The CD20 molecule is involved in regulation of B cell differentiation, except on plasma cells, it crosses the cell membrane four times, presumably via its reported function as a Ca++ channel subunit, B lymphocytes marker CD20 is a non glycosylated protein with a molecular weight of 35 or 37 kDa depending on the degree of phosphorylation
Purification methodThis antibody was purified via Protein A
Storage conditions 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA
Excitation Emission494nm/518nm
Synonyms B1 Bp35 MS4A2
Properties Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°
Gene targetCD20 Conjugated
Short name FITC Conjugated, CD20 Antibody
Technique Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody
LabelFITC
Alternative name fluorecein coupled, CD20 (Antibody to)
Alternative techniqueantibodies
Identity 7315
Gene MS4A1
Long gene name membrane spanning 4-domains A1
Synonyms gene CD20
Synonyms gene name member 1 , membrane-spanning 4-domains, subfamily A
Locus 11q12, 2
Discovery year 1989-05-23
GenBank acession M27394
Entrez gene record 931
Pubmed identfication 2448768
Classification CD molecules Membrane spanning 4-domains
Havana BLAST/BLAT OTTHUMG00000167614
Locus Specific Databases LRG_140

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