Filters

CD8 Antibody, FITC Conjugated

CD8 Antibody, FITC Conjugated size: 0.1ml 333

Price 333
Size 0.1ml
Long name FITC Conjugated, CD8 Polyclonal Antibody
CategoryConjugated Primary Antibodies
ConjugationAnti-FITC Antibody
Host OrganismRabbit (Oryctolagus cuniculus)
Target AntigenCD8
SpecificityThis is a highly specific antibody against CD8
ModificationUnmodified
Modification siteNone
ClonalityPolyclonal
ClonePolyclonal antibody
Concentration1ug per 1ul
Immunogen_range50-90/247
Subcellular locationExtracellular
SourceKLH conjugated synthetic peptide derived from mouse CD8
Gene ID number12525
Swiss ProtP01731
Tested applications IF(IHC-P), FCM
Recommended dilutions IF(IHC-P)(1:50-200), FCM(1:20-100)
Crossreactivity Rat, Mouse
Crossreactive species details not every possible cross-reactivity is known, Due to limited amount of testing and knowledge
Antigen background CD8 alpha chains binds to class I MHC molecules alpha-3 domains, CD8 is thought to play a role in the process of T-cell mediated killing, Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets
Purification methodThis antibody was purified via Protein A
Storage conditions 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA
Excitation Emission494nm/518nm
Properties Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°
Description This transmembrane glycoprotein is detected by monoclonals and has a lot of clones available with different affinity, Cytotoxic T cells cluster differentiators for flow cytometry
Gene targetCD8 Conjugated
Short name FITC Conjugated, CD8 Antibody
Technique Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody
LabelFITC
Alternative name fluorecein coupled, CD8 (Antibody to)
Alternative techniqueantibodies
Identity 1706
Gene CD8A
Long gene name CD8a molecule
Synonyms gene CD8
Synonyms gene name CD8 antigen, alpha polypeptide (p32) T-cell surface glycoprotein CD8 alpha chain
Locus 2p11, 2
Discovery year 1986-01-01
Entrez gene record 925
Pubmed identfication 1541829
RefSeq identity NM_001768
Classification CD molecules V-set domain containing
Havana BLAST/BLAT OTTHUMG00000130265
Locus Specific Databases CD8Abase: Mutation registry for CD8 deficiency LRG_44

Subscribe to our Newsletter