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cdc25A (Ser178) Antibody, FITC Conjugated

cdc25A (Ser178) Antibody, FITC Conjugated size: 0.1ml 350

Price 350
Size 0.1ml
Long name FITC Conjugated, cdc25A (Ser178) Polyclonal Antibody
CategoryConjugated Primary Antibodies
ConjugationAnti-FITC Antibody
Host OrganismRabbit (Oryctolagus cuniculus)
Target Antigencdc25A Ser178
SpecificityThis is a highly specific antibody against cdc25A Ser178
ModificationPhosphorylation
Modification siteSer178
ClonalityPolyclonal
ClonePolyclonal antibody
Concentration1ug per 1ul
SourceKLH conjugated synthetic phosphopeptide derived from human cdc25A around the phosphorylation site of Ser178 [QN(p-S)AP]
Gene ID number993
Tested applicationsIF(IHC-P)
Recommended dilutionsIF(IHC-P)(1:50-200)
Crossreactivity Mouse, Rat, Human
Crossreactive species details not every possible cross-reactivity is known, Due to limited amount of testing and knowledge
Antigen background CDC25B, CDC25C is predominantly expressed in G2, The activity of cyclin dependent kinases is also regulated by the phosphorylation status, These three genes function at different phases of the cell cycle, Three CDC25 genes are present in human cells: CDC25A, Whereas CDC25A and CDC25B are expressed throughout the cell cycle, and CDC25C, which is controlled by the antagonistic action of Wee1 kinase and CDC25 phosphatases, with peak expression in G1 for CDC25A and in both G1 S phase and G2 for CDC25B, Cyclin dependent kinases are positively regulated by association with cyclins and negatively regulated by binding to inhibitory subunits
Purification methodThis antibody was purified via Protein A
Storage conditions 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA
Excitation Emission494nm/518nm
Synonyms CDC25A2, Cell division cycle 25 homolog A S, Cell division cycle 25A, Cell division cycle 25A isoform a, Cell division cycle 25A isoform bv D9Ertd393ev Dual specicity phosphatase CDC25A, EC 3, M phase inducer phosphatase 1, MGC115549, pombe, 1, 3, 48, Cdc 25a
Properties Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°
Gene targetcdc25A (Ser178) Conjugated
Short name FITC Conjugated, cdc25A (Ser178) Antibody
Technique Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody
LabelFITC
Alternative name fluorecein coupled, cdc25A (Ser178) (Antibody to)
Alternative techniqueantibodies

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