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Cdc25B (Ser323) Antibody, FITC Conjugated

Cdc25B (Ser323) Antibody, FITC Conjugated size: 0.1ml 350

Price 350
Size 0.1ml
Long name FITC Conjugated, Cdc25B (Ser323) Polyclonal Antibody
CategoryConjugated Primary Antibodies
ConjugationAnti-FITC Antibody
Host OrganismRabbit (Oryctolagus cuniculus)
Target AntigenCdc25B Ser323
SpecificityThis is a highly specific antibody against Cdc25B Ser323
ModificationPhosphorylation
Modification siteSer323
ClonalityPolyclonal
ClonePolyclonal antibody
Concentration1ug per 1ul
Immunogen_range300-350/580
Subcellular locationCytoplasm
SourceKLH conjugated synthetic phosphopeptide derived from human Cdc25B around the phosphorylation site of Ser323
Gene ID number994
Swiss ProtP30305
Tested applicationsIF(IHC-P)
Recommended dilutionsIF(IHC-P)(1:50-200)
Crossreactivity Mouse, Rat, Human
Crossreactive species details not every possible cross-reactivity is known, Due to limited amount of testing and knowledge
Antigen background Directly dephosphorylates CDK1 and stimulates its kinase activity, Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner, The three isoforms seem to have a different level of activity, Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression
Purification methodThis antibody was purified via Protein A
Storage conditions 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA
Excitation Emission494nm/518nm
Synonyms CDC25B, CDC25HU2, Dual specificity phosphatase Cdc25B, M-phase inducer phosphatase 2
Properties Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°
Gene targetCdc25B (Ser323) Conjugated
Short name FITC Conjugated, Cdc25B (Ser323) Antibody
Technique Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody
LabelFITC
Alternative name fluorecein coupled, Cdc25B (Ser323) (Antibody to)
Alternative techniqueantibodies

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