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CDK2 (Thr14) Antibody, FITC Conjugated

CDK2 (Thr14) Antibody, FITC Conjugated size: 0.1ml 350

Supplier BIOSS PRIMARY CONJUGATED ANTIBODIES 1
Price 350
Size 0.1ml
Contact us Ask Component cell markers and DNA bp. markers are genetic markerser genes or DNA sequenceswith selection audit
Long name FITC Conjugated, CDK2 (Thr14) Polyclonal Antibody
CategoryConjugated Primary Antibodies
ConjugationAnti-FITC Antibody
Host OrganismRabbit (Oryctolagus cuniculus)
Target AntigenCDK2 Thr14
SpecificityThis is a highly specific antibody against CDK2 Thr14
ModificationPhosphorylation
Modification siteThr14
ClonalityPolyclonal
ClonePolyclonal antibody
Concentration1ug per 1ul
SourceKLH conjugated synthetic phosphopeptide derived from human CDK2 around the phosphorylation site of Thr14 [EG(p-T)YG]
Gene ID number1017
Tested applicationsIF(IHC-P)
Recommended dilutionsIF(IHC-P)(1:50-200)
Crossreactivity Mouse, Rat, Human
Crossreactive species details not every possible cross-reactivity is known, Due to limited amount of testing and knowledge
Antigen background CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B), It is a catalytic subunit of the cyclin-dependent protein kinase complex, Its activity is also regulated by its protein phosphorylation, This protein associates with and regulated by the regulatory subunits of the complex including cyclin A or E, This protein kinase is highly similar to the gene products of S, Two alternatively spliced variants and multiple transcription initiation sites of this gene have been reported, and S, and essential for cell cycle G1/S phase transition, cdk2 binds cyclin type A and E proteins and controls progression into S-phase, cdk2 is a cell cycle protein closely related to Cdc2 (cdk1) that has proved useful as a marker of proliferation, cerevisiae cdc28, pombe cdc2, whose activity is restricted to the G1-S phase, The protein encoded by this gene is a member of the Ser/Thr protein kinase family
Purification methodThis antibody was purified via Protein A
Storage conditions 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA
Excitation Emission494nm/518nm
Properties Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°
Gene targetCDK2 (Thr14) Conjugated
Short name FITC Conjugated, CDK2 (Thr14) Antibody
Technique Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody
LabelFITC
Alternative name fluorecein coupled, CDK2 (Thr14) (Antibody to)
Alternative techniqueantibodies
Identity 1771
Gene CDK2
Long gene name cyclin dependent kinase 2
Locus 12q13, 2
Discovery year 1992-02-28
GenBank acession M68520
Entrez gene record 1017
Pubmed identfication 1717994 8275715
Classification Cyclin dependent kinases
Havana BLAST/BLAT OTTHUMG00000170575

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