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CENPB Antibody, Biotin Conjugated

CENPB Antibody, Biotin Conjugated size: 0.1ml 333

Price 333
Size 0.1ml
Long name Biotin Conjugated, CENPB Polyclonal Antibody
CategoryConjugated Primary Antibodies
ConjugationBiotinylated
Host OrganismRabbit (Oryctolagus cuniculus)
Target AntigenCENPB
SpecificityThis is a highly specific antibody against CENPB
ModificationUnmodified
Modification siteNone
ClonalityPolyclonal
ClonePolyclonal antibody
Concentration1ug per 1ul
Subcellular locationNucleus
SourceKLH conjugated synthetic peptide derived from human CENPB
Gene ID number1059
Tested applications IHC-P, WB
Recommended dilutions IHC-P(1:100-500), WB(1:100-1000)
Crossreactivity Mouse, Rat, Human
Crossreactive species details not every possible cross-reactivity is known, Due to limited amount of testing and knowledge
Antigen background (referenced from Entrez Gene), It is a DNA-binding protein containing a helix loop helix DNA binding motif at the N-terminus, It is also considered a major centromere autoantigen recognized by sera from patients with anti-centromere , The DNA binding domain recognizes and binds a 17 bp sequence (CENPB box) in the centromeric satellite DNA, This protein is proposed to play an important role in the assembly of specific centromere structures in interphase nuclei and on mitotic chromosomes, and a dimerization domain at the C-terminus, This gene product is a highly conserved protein associated with the centromere
Purification methodThis antibody was purified via Protein A
Storage conditions 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA
Excitation EmissionN/A
Properties Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, Biotin conjugates can be detected by horseradish peroxidase, To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, alkaline phosphatase substrates or anti biotin conjugated antibodies, 6, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°
Gene targetCENPB Conjugated
Short name Conjugated, CENPB Antibody
Technique antibodies against human proteins, antibodies for, antibody Conjugates, Antibody
LabelBiotin
Alternative name biotinilated coupled, CENPB (Antibody to)
Alternative techniqueantibodies
Identity 1852
Gene CENPB
Long gene name centromere protein B
Synonyms gene name 80kDa , centromere protein B (80kD) centromere protein B
Locus 20p13
Discovery year 1988-05-31
GenBank acession X05299
Entrez gene record 1059
Pubmed identfication 8406460 11884609
RefSeq identity NM_001810
Classification Helix-turn-helix CENPB type domain containing
Havana BLAST/BLAT OTTHUMG00000031761

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