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Ceramide Kinase (CERK) ELISA kit

Ceramide Kinase (CERK) ELISA kit size: 1 plate of 96 wells 822

Supplier BIOMATIK ELISA KITS
Price 822
Size 1 plate of 96 wells
Contact us Ask Component cell markers and DNA bp. markers are genetic markerser genes or DNA sequenceswith selection audit
Antigen detectable with this ELISA kitCeramide Kinase (CERK)
Species reactivityHomo sapiens (Human)
Range of detection 100ng/mL, 12, 25ng/mL, 3, 50ng/mL, 6, 125-200ng/mL The standard curve concentrations used for the ELISA&rsquo, 125ng/mL, 25ng/mL, 3, 5ng/mL, s were 200ng/mL
Sensitivity44ng/mL, The minimum detectable dose of this kit is typically less than 1
ApplicationELISA
Sample types tested for this ELISA kit cell lysates and other biological fluids, Tissue homogenates
Time for performing t hourse test4, 5 hoursours
Test principle 10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ceramide Kinase (CERK), The concentration of Ceramide Kinase (CERK) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ceramide Kinase (CERK), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Ceramide Kinase (CERK), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn
Storage and handling Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt
Advisory Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes
Properties E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
TestELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Gene targetCeramide Kinase (CERK)
Short nameCeramide Kinase (CERK) ELISA kit
Technique &hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit
Hostantigen assay ELISA kit 1 Kit (96 Wells)
Alternative nameCeramide phosphorylation catalyst (ceramide kinase) Enzyme-linked immunosorbent assay reagent
Alternative techniqueelisas
Alternative to gene target ATP binding, CERK and IDBG-12219 and ENSG00000100422 and 64781, CERK and IDBG-642451 and ENSBTAG00000046654 and, Cerk and IDBG-171293 and ENSMUSG00000035891 and 223753, Plasma membranes, this GO :0000287 and magnesium ion binding and molecular function this GO :0001729 and ceramide kinase activity and molecular function this GO :0003951 and NAD+ kinase activity and molecular function this GO :0004143 and diacylglycerol kinase activity and molecular function this GO :0005524 and ATP binding and molecular function this GO :0005739 and mitochondrion and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0006665 and sphin this GO lipid metabolic process and biological process this GO :0006672 and ceramide metabolic process and biological process this GO :0006687 and glycosphin this GO lipid metabolic process and biological process this GO :0007205 and protein kinase C-activating G-protein coupled receptor signaling pathway and biological process this GO :0008152 and metabolic process and biological process this GO :0016021 and integral component of membrane and cellular component this GO :0016310 and phosphorylation and biological process this GO :0044281 and small molecule metabolic process and biological process this GO :0046834 and lipid phosphorylation and biological process, this GO :0000287: magnesium ion binding, this GO :0000287: magnesium ion binding and also this GO :0001729: ceramide kinase activity and also this GO :0003951: NAD+ kinase activity and also this GO :0004143: diacylglycerol kinase activity and also this GO :0005524: ATP binding, this GO :0001729: ceramide kinase activity, this GO :0003951: NAD+ kinase activity, this GO :0004143: diacylglycerol kinase activity, this GO :0005524: ATP binding, ceramide kinase
Identity 19256
Gene CERK
Long gene name ceramide kinase
Synonyms hCERK FLJ23239 dA59H18, 2 , 3 DKFZp434E0211 FLJ21430 KIAA1646 LK4 dA59H18
Locus 22q13, 31
Discovery year 2002-10-09
GenBank acession AB079066
Entrez gene record 64781
Pubmed identfication 11956206 11258795
RefSeq identity NM_022766
Havana BLAST/BLAT OTTHUMG00000150395

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