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CHEK1(Ser317) Antibody

CHEK1(Ser317) Antibody size: 0.1ml 281

Supplier BIOSS PRIMARY UNCONJUGATED ANTIBODIES
Price 281
Size 0.1ml
Contact us Ask Component cell markers and DNA bp. markers are genetic markerser genes or DNA sequenceswith selection audit
Long nameCHEK1(Ser317) Primary Polyclonal Antibody
Also known asCHEK1(Ser317) PAb
CategoryPrimary Antibodies
ConjugationUnconjugated
Target AntigenCHEK1 Ser317
Specificity This phosphorylation site is homologous to that of Ser317 in Mouse and Rat, This is a highly specific antibody against CHEK1 Ser317
Modification(s)Phosphorylation
Modification site(s)Ser317
ClonalityPolyclonal antibody
Clone numberPolyclonal antibody
Concentration1ug per 1ul
Immunogen range280-330/476
Subcellular location Nucleus, Cytoplasm
SourceKLH conjugated synthetic phosphopeptide derived from human CHEK1 around the phosphorylation site of Ser317
Gene ID number1111
Swiss ProtO14757
Tested Applications IF(IHC-P), IHC-P, WB
Recommended dilutions IF(IHC-P)(1:50-200), IHC-P(1:100-500), WB(1:100-1000)
Cross reactivity Mouse, Rat, Human
Cross reactive species details not every possible cross-reactivity is known, Due to limited amount of testing and knowledge
Background of the target antigen 'Ser-124', 'Ser-178', 'Ser-279' and 'Ser-293' promotes proteolysis of CDC25A, 'Ser-82' and 'Ser-88' by NEK11, Also phosphorylates NEK6, Also promotes repair of DNA cross-links through phosphorylation of FANCE, Binds to and phosphorylates CDC25A, Binds to and phosphorylates RAD51 at 'Thr-309', Binds to and phosphorylates TLK1 at 'Ser-743', CDC25B and CDC25C, Inhibition of CDC25 leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes and blocks cell cycle progression, May also negatively regulate cell cycle progression during unperturbed cell cycles, May also play a role in replication fork maintenance through regulation of PCNA, Phosphorylates multiple sites within the C-terminus of TP53, Phosphorylation of CDC25A at 'Ser-178' and 'Thr-507' and phosphorylation of CDC25C at 'Ser-216' creates binding sites for 14-3-3 proteins which inhibit CDC25A and CDC25C, Phosphorylation of CDC25A at 'Ser-76', Phosphorylation of CDC25A at 'Ser-76' primes the protein for subsequent phosphorylation at 'Ser-79', Recognizes the substrate consensus sequence [R-X-X-S/T], This may enhance chromatin assembly both in the presence or absence of DNA damage, This regulation is achieved by a number of mechanisms that together help to preserve the integrity of the genome, thereby promoting DNA repair by homologous recombination, which is required for polyubiquitination and degradation of CDCD25A, which prevents the TLK1-dependent phosphorylation of the chromatin assembly factor ASF1A, which promotes activation of TP53 by acetylation and promotes cell cycle arrest and suppression of cellular proliferation, which promotes the release of RAD51 from BRCA2 and enhances the association of RAD51 with chromatin, Serine/threonine-protein kinase which is required for checkpoint-mediated cell cycle arrest and activation of DNA repair in response to the presence of DNA damage or unreplicated DNA
Purification methodThis antibody was purified via Protein A
Storage conditions 50% glycerol and 0, Store at -20°, 09% sodium azide, C for up to 1 year, Keep the antibody in aqueous buffered solution containing 1% BSA
Synonym names CHEK1, CHK1 checkpoint homolog, Cell cycle checkpoint kinase, Checkpoint kinase-1, Serine/threonine-protein kinase Chk1, CHK1
PropertiesC, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Unconjugated Antibodies they should be stored frozen at - 24°
Gene targetCHEK1(Ser317)
Short nameCHEK1(Ser317) Antibody
Technique antibodies against human proteins, antibodies for, Antibody
Alternative nameCHEK1(Ser317) (Antibody to)
Alternative techniqueantibodies
Identity 1925
Gene CHEK1
Long gene name checkpoint kinase 1
Synonyms gene name CHK1 (checkpoint, S, pombe) , pombe) homolog CHK1 checkpoint homolog (S
Synonyms CHK1
Locus 11q24, 2
Discovery year 1998-04-21
GenBank acession AF016582 BC017575
Entrez gene record 1111
Pubmed identfication 9278511 9382850
RefSeq identity NM_001274
Havana BLAST/BLAT OTTHUMG00000165853

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