| Long name | CHK2 Primary Polyclonal Antibody |
| Also known as | CHK2 PAb |
| Category | Primary Antibodies |
| Conjugation | Unconjugated |
| Target Antigen | CHK2 |
| Specificity | This is a highly specific antibody against CHK2 |
| Modification(s) | None |
| Modification site(s) | Unmodified antibody |
| Clonality | Polyclonal antibody |
| Clone number | Polyclonal antibody |
| Concentration | 1ug per 1ul |
| Immunogen range | 120-160/543 |
| Subcellular location | Nucleus |
| Source | This antibody was obtained by immunization of the host with KLH conjugated synthetic peptide derived from human CHK2 |
| Gene ID number | 11200 |
| Swiss Prot | O96017 |
| Tested Applications | IF(IHC-P), IHC-P, WB |
| Recommended dilutions | IF(IHC-P)(1:50-200), IHC-P(1:100-500), WB(1:100-1000) |
| Cross reactivity | Mouse, Rat, Human |
| Cross reactive species details | not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Background of the target antigen | Also controls the transcription of pro-apoptotic genes through phosphorylation of the transcription factor E2F1, Also stimulates the transcription of genes involved in DNA repair (including BRCA2) through the phosphorylation and activation of the transcription factor FOXM1, CDC25B and CDC25C, Following activation, Inhibition of CDC25 phosphatase activity leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes and blocks cell cycle progression, Its absence may be a cause of the chromosomal instability observed in some cancer cells, MDM4 and PML, May also negatively regulate cell cycle progression during unperturbed cell cycles, May also phosphorylate NEK6 which is involved in G2/M cell cycle arrest, Phosphorylation of MDM4 may also reduce degradation of p53/TP53, Phosphorylation of p53/TP53 at 'Ser-20' by CHEK2 may alleviate inhibition by MDM2, Regulates DNA repair through phosphorylation of BRCA2, Regulates apoptosis through the phosphorylation of p53/TP53, Regulates cell cycle checkpoint arrest through phosphorylation of CDC25A, Tumor suppressor, activation of DNA repair and apoptosis in response to the presence of DNA double-strand breaks, enhancing the association of RAD51 with chromatin which promotes DNA repair by homologous recombination, inhibiting their activity, it may also have a DNA damage-independent function in mitotic spindle assembly by phosphorylating BRCA1, leading to accumulation of active p53/TP53, phosphorylates numerous effectors preferentially at the consensus sequence [L-X-R-X-X-S/T], Serine/threonine-protein kinase which is required for checkpoint-mediated cell cycle arrest |
| Purification method | This antibody was purified via Protein A |
| Storage conditions | 50% glycerol and 0, Store at -20°, 09% sodium azide, C for up to 1 year, Keep the antibody in aqueous buffered solution containing 1% BSA |
| Synonym names | CHEK2, CHK2, CHK2 checkpoint homolog, Cds1 homolog, Checkpoint kinase 2, HuCds1, LFS2, PP1425, RAD53, Serine/threonine-protein kinase Chk2, hCds1, CDS1 |
| Properties | C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Unconjugated Antibodies they should be stored frozen at - 24° |
| Gene target | CHK2 |
| Short name | CHK2 Antibody |
| Technique | antibodies against human proteins, antibodies for, Antibody |
| Alternative name | CHK2 (Antibody to) |
| Alternative technique | antibodies |
| Identity | 16627 |
| Gene | CHEK2 |
| Long gene name | checkpoint kinase 2 |
| Synonyms gene | RAD53 |
| Synonyms gene name | CHK2 (checkpoint, S, pombe) , pombe) homolog CHK2 checkpoint homolog (S |
| Synonyms | CDS1 CHK2 HuCds1 PP1425 bA444G7 |
| Locus | 22q12, 1 |
| Discovery year | 2001-09-19 |
| GenBank acession | AF086904 |
| Entrez gene record | 11200 |
| Pubmed identfication | 9836640 10097108 |
| RefSeq identity | NM_001005735 |
| Havana BLAST/BLAT | OTTHUMG00000151023 |
| Locus Specific Databases | LRG_302 |
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