Filters

CHK2 Antibody, FITC Conjugated

CHK2 Antibody, FITC Conjugated size: 0.1ml 333

Supplier BIOSS PRIMARY CONJUGATED ANTIBODIES 1
Price 333
Size 0.1ml
Contact us Ask Component cell markers and DNA bp. markers are genetic markerser genes or DNA sequenceswith selection audit
Long name FITC Conjugated, CHK2 Polyclonal Antibody
CategoryConjugated Primary Antibodies
ConjugationAnti-FITC Antibody
Host OrganismRabbit (Oryctolagus cuniculus)
Target AntigenCHK2
SpecificityThis is a highly specific antibody against CHK2
ModificationUnmodified
Modification siteNone
ClonalityPolyclonal
ClonePolyclonal antibody
Concentration1ug per 1ul
Immunogen_range120-160/543
Subcellular locationNucleus
SourceKLH conjugated synthetic peptide derived from human CHK2
Gene ID number11200
Swiss ProtO96017
Tested applicationsIF(IHC-P)
Recommended dilutionsIF(IHC-P)(1:50-200)
Crossreactivity Mouse, Rat, Human
Crossreactive species details not every possible cross-reactivity is known, Due to limited amount of testing and knowledge
Antigen background Also controls the transcription of pro-apoptotic genes through phosphorylation of the transcription factor E2F1, Also stimulates the transcription of genes involved in DNA repair (including BRCA2) through the phosphorylation and activation of the transcription factor FOXM1, CDC25B and CDC25C, Following activation, Inhibition of CDC25 phosphatase activity leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes and blocks cell cycle progression, Its absence may be a cause of the chromosomal instability observed in some cancer cells, MDM4 and PML, May also negatively regulate cell cycle progression during unperturbed cell cycles, May also phosphorylate NEK6 which is involved in G2/M cell cycle arrest, Phosphorylation of MDM4 may also reduce degradation of p53/TP53, Phosphorylation of p53/TP53 at 'Ser-20' by CHEK2 may alleviate inhibition by MDM2, Regulates DNA repair through phosphorylation of BRCA2, Regulates apoptosis through the phosphorylation of p53/TP53, Regulates cell cycle checkpoint arrest through phosphorylation of CDC25A, Tumor suppressor, activation of DNA repair and apoptosis in response to the presence of DNA double-strand breaks, enhancing the association of RAD51 with chromatin which promotes DNA repair by homologous recombination, inhibiting their activity, it may also have a DNA damage-independent function in mitotic spindle assembly by phosphorylating BRCA1, leading to accumulation of active p53/TP53, phosphorylates numerous effectors preferentially at the consensus sequence [L-X-R-X-X-S/T], Serine/threonine-protein kinase which is required for checkpoint-mediated cell cycle arrest
Purification methodThis antibody was purified via Protein A
Storage conditions 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA
Excitation Emission494nm/518nm
Synonyms CDS1 CHK2 HuCds1 PP1425 bA444G7
Properties Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°
Gene targetCHK2 Conjugated
Short name FITC Conjugated, CHK2 Antibody
Technique Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody
LabelFITC
Alternative name fluorecein coupled, CHK2 (Antibody to)
Alternative techniqueantibodies
Identity 16627
Gene CHEK2
Long gene name checkpoint kinase 2
Synonyms gene RAD53
Synonyms gene name CHK2 (checkpoint, S, pombe) , pombe) homolog CHK2 checkpoint homolog (S
Locus 22q12, 1
Discovery year 2001-09-19
GenBank acession AF086904
Entrez gene record 11200
Pubmed identfication 9836640 10097108
RefSeq identity NM_001005735
Havana BLAST/BLAT OTTHUMG00000151023
Locus Specific Databases LRG_302

Similar products

Subscribe to our Newsletter