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CHK2 (Thr387) Antibody, FITC Conjugated

CHK2 (Thr387) Antibody, FITC Conjugated size: 0.1ml 350

Supplier BIOSS PRIMARY CONJUGATED ANTIBODIES 1
Price 350
Size 0.1ml
Contact us Ask Component cell markers and DNA bp. markers are genetic markerser genes or DNA sequenceswith selection audit
Long name FITC Conjugated, CHK2 (Thr387) Polyclonal Antibody
CategoryConjugated Primary Antibodies
ConjugationAnti-FITC Antibody
Host OrganismRabbit (Oryctolagus cuniculus)
Target AntigenCHK2 Thr387
SpecificityThis is a highly specific antibody against CHK2 Thr387
ModificationPhosphorylation
Modification siteThr387
ClonalityPolyclonal
ClonePolyclonal antibody
Concentration1ug per 1ul
SourceKLH conjugated synthetic phosphopeptide derived from human CHK2 around the phosphorylation site of Thr387
Gene ID number11200
Tested applicationsIF(IHC-P)
Recommended dilutionsIF(IHC-P)(1:50-200)
Crossreactivity Mouse, Rat, Human
Crossreactive species details not every possible cross-reactivity is known, Due to limited amount of testing and knowledge
Antigen background Chk2 inhibits CDC25C phosphatase by phosphorylating it on Ser-216, Chk2 is the mammalian ortholog of the budding yeast Rad53 and fission yeast Cds1 checkpoint kinases, Indeed, Kinase activity is up regulated by autophosphorylation and the protein is rapidly phosphorylated in response to DNA damage and to replication block, Phosphorylation at Thr68 is a prerequisite for the subsequent activation step, Ser28, Ser33, Ser35, Ser50 and Thr68) followed by glutamine (SQ or TQ motif), The SQ/TQ cluster domain, The amino-terminal domain of Chk2 contains a series of seven serine and threonine residues (Ser19, These are known to be preferred sites for phosphorylation by ATM/ATR kinases, This kinase may have a role in meiosis as well, Thr26, Thr68 and other sites in this region become phosphorylated by ATM/ATR, UV irradiation or hydroxyurea treatment, after DNA damage by ionizing radiation (IR), and may also participate in transduction of the DNA damage and replicational stress signals, preventing the entry into mitosis, seems to have a regulatory function, therefore, which is attributable to autophosphorylation of Chk2 on residues Thr383 and Thr387 in the activation loop of the kinase domain, Chk2 is a serine/threonine kinase involved in the control of cell cycle checkpoints
Purification methodThis antibody was purified via Protein A
Storage conditions 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA
Excitation Emission494nm/518nm
Synonyms CDS1 CHK2 HuCds1 PP1425 bA444G7
Properties Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°
Gene targetCHK2 (Thr387) Conjugated
Short name FITC Conjugated, CHK2 (Thr387) Antibody
Technique Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody
LabelFITC
Alternative name fluorecein coupled, CHK2 (Thr387) (Antibody to)
Alternative techniqueantibodies
Identity 16627
Gene CHEK2
Long gene name checkpoint kinase 2
Synonyms gene RAD53
Synonyms gene name CHK2 (checkpoint, S, pombe) , pombe) homolog CHK2 checkpoint homolog (S
Locus 22q12, 1
Discovery year 2001-09-19
GenBank acession AF086904
Entrez gene record 11200
Pubmed identfication 9836640 10097108
RefSeq identity NM_001005735
Havana BLAST/BLAT OTTHUMG00000151023
Locus Specific Databases LRG_302

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