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Collagen IV Antibody, Biotin Conjugated

Collagen IV Antibody, Biotin Conjugated size: 0.1ml 333

Price 333
Size 0.1ml
Long name Biotin Conjugated, Collagen IV Polyclonal Antibody
CategoryConjugated Primary Antibodies
ConjugationBiotinylated
Host OrganismRabbit (Oryctolagus cuniculus)
Target AntigenCollagen IV
Specificity This antibody has 79% cross reactivity with Collagen alpha-5(IV) chain, This is a highly specific antibody against Collagen IV
ModificationUnmodified
Modification siteNone
ClonalityPolyclonal
ClonePolyclonal antibody
Concentration1ug per 1ul
Immunogen_range1430-1480/1669
Subcellular locationSecreted
SourceKLH conjugated synthetic peptide derived from mouse Collagen alpha-1(IV) chain
Gene ID number12826
Swiss ProtP02463
Tested applications IHC-P, WB
Recommended dilutions IHC-P(1:100-500), WB(1:100-1000)
Crossreactivity Mouse, Rat, Human
Crossreactive species details not every possible cross-reactivity is known, Due to limited amount of testing and knowledge
Antigen background Arresten, Inhibits expression of hypoxia-inducible factor 1alpha and ERK1/2 and p38 MAPK activation, Ligand for alpha1/beta1 integrin (By similarity), Specifically inhibits endothelial cell proliferation, The C-terminal half is found to possess the anti-angiogenic activity, comprising the C-terminal NC1 domain, forming a 'chicken-wire' meshwork together with laminins, inhibits angiogenesis and tumor formation, migration and tube formation, proteoglycans and entactin/nidogen, Type IV collagen is the major structural component of glomerular basement membranes (GBM)
Purification methodThis antibody was purified via Protein A
Storage conditions 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA
Excitation EmissionN/A
Synonyms Col4a-1, Col4a1, Collagen alpha-1(IV) chain, Del(8)44H, Raw, Svc, Bru
Properties Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, Biotin conjugates can be detected by horseradish peroxidase, To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, alkaline phosphatase substrates or anti biotin conjugated antibodies, 6, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°
Gene targetCollagen IV Conjugated
Short name Conjugated, Collagen IV Antibody
Technique antibodies against human proteins, antibodies for, antibody Conjugates, Antibody
LabelBiotin
Alternative name biotinilated coupled, Collagen IV (Antibody to)
Alternative techniqueantibodies

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