Name : Crk II (Tyr221) Antibody, Biotin Conjugated
Supplier : BIOSS PRIMARY CONJUGATED ANTIBODIES 1
Price :350
SKU : GEN5979985682
| Long name | Biotin Conjugated, Crk II (Tyr221) Polyclonal Antibody |
| Category | Conjugated Primary Antibodies |
| Conjugation | Biotinylated |
| Host Organism | Rabbit (Oryctolagus cuniculus) |
| Target Antigen | Crk isoform II Tyr221 |
| Specificity | This is a highly specific antibody against Crk isoform II Tyr221 |
| Modification | Phosphorylation |
| Modification site | Tyr221 |
| Clonality | Polyclonal |
| Clone | Polyclonal antibody |
| Concentration | 1ug per 1ul |
| Source | KLH conjugated synthetic phosphopeptide derived from human Crk p38 around the phosphorylation site of Tyr221 [GP(p-Y)AQ] |
| Gene ID number | 1398 |
| Tested applications | IHC-P, WB |
| Recommended dilutions | IHC-P(1:100-500), WB(1:100-1000) |
| Crossreactivity | Mouse, Rat, Human |
| Crossreactive species details | not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background | Crk-II has less transforming activity than Crk-I, Crk-II mediates attachment-induced MAPK8 activation, Involved in phagocytosis of apoptotic cells and cell motility via its interaction with DOCK1 and DOCK4, May regulate the EFNA5-EPHA3 signaling, membrane ruffling and cell motility in a Rac-dependent manner, The Crk-I and Crk-II forms differ in their biological activities |
| Purification method | This antibody was purified via Protein A |
| Storage conditions | 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission | N/A |
| Synonyms | CRK, Proto-oncogene c-Crk, p38, Adapter molecule crk |
| Properties | Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, Biotin conjugates can be detected by horseradish peroxidase, To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, alkaline phosphatase substrates or anti biotin conjugated antibodies, 6, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target | Crk II (Tyr221) Conjugated |
| Short name | Conjugated, Crk II (Tyr221) Antibody |
| Technique | antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label | Biotin |
| Alternative name | biotinilated coupled, Crk II (Tyr221) (Antibody to) |
| Alternative technique | antibodies |