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Crk II (Tyr221) Antibody, Biotin Conjugated

Crk II (Tyr221) Antibody, Biotin Conjugated size: 0.1ml 350

Price 350
Size 0.1ml
Long name Biotin Conjugated, Crk II (Tyr221) Polyclonal Antibody
CategoryConjugated Primary Antibodies
ConjugationBiotinylated
Host OrganismRabbit (Oryctolagus cuniculus)
Target AntigenCrk isoform II Tyr221
SpecificityThis is a highly specific antibody against Crk isoform II Tyr221
ModificationPhosphorylation
Modification siteTyr221
ClonalityPolyclonal
ClonePolyclonal antibody
Concentration1ug per 1ul
SourceKLH conjugated synthetic phosphopeptide derived from human Crk p38 around the phosphorylation site of Tyr221 [GP(p-Y)AQ]
Gene ID number1398
Tested applications IHC-P, WB
Recommended dilutions IHC-P(1:100-500), WB(1:100-1000)
Crossreactivity Mouse, Rat, Human
Crossreactive species details not every possible cross-reactivity is known, Due to limited amount of testing and knowledge
Antigen background Crk-II has less transforming activity than Crk-I, Crk-II mediates attachment-induced MAPK8 activation, Involved in phagocytosis of apoptotic cells and cell motility via its interaction with DOCK1 and DOCK4, May regulate the EFNA5-EPHA3 signaling, membrane ruffling and cell motility in a Rac-dependent manner, The Crk-I and Crk-II forms differ in their biological activities
Purification methodThis antibody was purified via Protein A
Storage conditions 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA
Excitation EmissionN/A
Synonyms CRK, Proto-oncogene c-Crk, p38, Adapter molecule crk
Properties Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, Biotin conjugates can be detected by horseradish peroxidase, To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, alkaline phosphatase substrates or anti biotin conjugated antibodies, 6, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°
Gene targetCrk II (Tyr221) Conjugated
Short name Conjugated, Crk II (Tyr221) Antibody
Technique antibodies against human proteins, antibodies for, antibody Conjugates, Antibody
LabelBiotin
Alternative name biotinilated coupled, Crk II (Tyr221) (Antibody to)
Alternative techniqueantibodies

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