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CSTF3/CSTF77 Antibody, Biotin Conjugated

CSTF3/CSTF77 Antibody, Biotin Conjugated size: 0.1ml 333

Price 333
Size 0.1ml
Long name Biotin Conjugated, CSTF3/CSTF77 Polyclonal Antibody
CategoryConjugated Primary Antibodies
ConjugationBiotinylated
Host OrganismRabbit (Oryctolagus cuniculus)
Target AntigenCSTF3/CSTF77
SpecificityThis is a highly specific antibody against CSTF3/CSTF77
ModificationUnmodified
Modification siteNone
ClonalityPolyclonal
ClonePolyclonal antibody
Concentration1ug per 1ul
SourceKLH conjugated synthetic peptide derived from human CSTF3/CSTF77
Gene ID number1479
Tested applications IHC-P, WB
Recommended dilutions IHC-P(1:100-500), WB(1:100-1000)
Crossreactivity Mouse, Rat, Human
Crossreactive species details not every possible cross-reactivity is known, Due to limited amount of testing and knowledge
Antigen background 77 kDa subunit), CstF-77 (cleavage stimulation factor, CstF-77 is capable of interacting with CPSF1 and FCP1, CstF-77 is highly conserved among eukaryotes, Heterotrimeric CstF recognizes GU- and U-rich sequences located downstream of the polyadenylation site on RNA, In addition, It can exist as a homodimer and functions as the bridge, It contains an Alpha-helical structure with 11 HAT (Half-a-TPR-containing) repeats and is essential for CstF assembly, The first step, a heterotrimer that is composed of three distinct subunits, also known as CstF3, directly interacting with the other two CstF subunits, endonucleolytic cleavage of polyadenylation substrates, is one of the three subunits comprising CstF, namely CstF-64 and CstF-50, other factors involved in polyadenylation, requires CstF (cleavage stimulation factor), Polyadenylation of mRNA precursors is a two-step reaction that requires multiple protein factors
Purification methodThis antibody was purified via Protein A
Storage conditions 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA
Excitation EmissionN/A
Synonyms CstF-77
Properties Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, Biotin conjugates can be detected by horseradish peroxidase, To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, alkaline phosphatase substrates or anti biotin conjugated antibodies, 6, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°
Gene targetCSTF3/CSTF77 Conjugated
Short name Conjugated, CSTF3/CSTF77 Antibody
Technique antibodies against human proteins, antibodies for, antibody Conjugates, Antibody
LabelBiotin
Alternative name biotinilated coupled, CSTF3/CSTF77 (Antibody to)
Alternative techniqueantibodies
Identity 2485
Gene CSTF3
Long gene name cleavage stimulation factor subunit 3
Synonyms gene name 3', 3', 77kD cleavage stimulation factor, cleavage stimulation factor, pre-RNA, pre-RNA, subunit 3, subunit 3
Locus 11p13
Discovery year 1995-01-16
GenBank acession U15782
Entrez gene record 1479
Pubmed identfication 7984242
RefSeq identity NM_001326
Havana BLAST/BLAT OTTHUMG00000166268

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