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Cyclin D1 Antibody, Biotin Conjugated

Cyclin D1 Antibody, Biotin Conjugated size: 0.1ml 333

Supplier BIOSS PRIMARY CONJUGATED ANTIBODIES 1
Price 333
Size 0.1ml
Contact us Ask Component cell markers and DNA bp. markers are genetic markerser genes or DNA sequenceswith selection audit
Long name Biotin Conjugated, Cyclin D1 Polyclonal Antibody
CategoryConjugated Primary Antibodies
ConjugationBiotinylated
Host OrganismRabbit (Oryctolagus cuniculus)
Target AntigenCyclin D1
SpecificityThis is a highly specific antibody against Cyclin D1
ModificationUnmodified
Modification siteNone
ClonalityPolyclonal
ClonePolyclonal antibody
Concentration1ug per 1ul
Immunogen_range70-120/295
Subcellular location Nucleus, Cytoplasm
SourceKLH conjugated synthetic peptide derived from mouse Cyclin D1
Gene ID number12443
Swiss ProtP25322
Tested applications IHC-P, WB
Recommended dilutions IHC-P(1:100-500), WB(1:100-1000)
Crossreactivity Goat, Mouse, Rat, Human
Crossreactive species details not every possible cross-reactivity is known, Due to limited amount of testing and knowledge
Antigen background Also substrate for SMAD3, Component of the ternary complex, Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals, Exhibits transcriptional corepressor activity with INSM1 on the NEUROD1 and INS promoters in a cell cycle-independent manner (By similarity), Hypophosphorylates RB1 in early G(1) phase, Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase, cyclin D1/CDK4/CDKN1B, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity, required for nuclear translocation and activity of the cyclin D-CDK4 complex, Regulatory component of the cyclin D1-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition
Purification methodThis antibody was purified via Protein A
Storage conditions 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA
Excitation EmissionN/A
Synonyms AI32739, Ccnd1, CycD1, Cyl-1, G1/S-specific cyclin-D1, PRAD1, bcl-1, cD1
Properties Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, Biotin conjugates can be detected by horseradish peroxidase, To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, alkaline phosphatase substrates or anti biotin conjugated antibodies, 6, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°
Gene targetCyclin D1 Conjugated
Short name Conjugated, Cyclin D1 Antibody
Technique antibodies against human proteins, antibodies for, antibody Conjugates, Antibody
LabelBiotin
Alternative name biotinilated coupled, Cyclin D1 (Antibody to)
Alternative techniqueantibodies

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