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Cyclin E Antibody, FITC Conjugated

Cyclin E Antibody, FITC Conjugated size: 0.1ml 333

Supplier BIOSS PRIMARY CONJUGATED ANTIBODIES 1
Price 333
Size 0.1ml
Contact us Ask Component cell markers and DNA bp. markers are genetic markerser genes or DNA sequenceswith selection audit
Long name FITC Conjugated, Cyclin E Polyclonal Antibody
CategoryConjugated Primary Antibodies
ConjugationAnti-FITC Antibody
Host OrganismRabbit (Oryctolagus cuniculus)
Target AntigenCyclin E
SpecificityThis is a highly specific antibody against Cyclin E
ModificationUnmodified
Modification siteNone
ClonalityPolyclonal
ClonePolyclonal antibody
Concentration1ug per 1ul
Immunogen_range370-411/411
Subcellular locationNucleus
SourceKLH conjugated synthetic peptide derived from rat Cyclin E
Gene ID number25729
Swiss ProtP39949
Tested applications IF(IHC-P), FCM
Recommended dilutions IF(IHC-P)(1:50-200), FCM(1:20-100)
Crossreactivity Rat, Mouse
Crossreactive species details not every possible cross-reactivity is known, Due to limited amount of testing and knowledge
Antigen background Cyclins function as regulators of CDK kinases, Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event, Overexpression of this gene has been observed in many tumors, The encoded protein has a 15 aa longer N-terminus, This cyclin forms a complex with and functions as a regulatory subunit of CDK2, This protein accumulates at the G1-S phase boundary and is degraded as cells progress through S phase, This protein was found to associate with, Transcript Variant: This variant (1) contains a different 5' end region, Two additional splice variants were reported but detailed nucleotide sequence information is not yet available, Two alternatively spliced transcript variants of this gene, and be involved in, and thus may contribute to tumorigenesis, as compared to isoform 2, have been described, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), when compared to variant 2, which encode distinct isoforms, which includes an upstream in-frame translation start codon, which participates in cell-cycle regulated histone gene expression and plays a critical role in promoting cell-cycle progression in the absence of pRB, which results in chromosome instability, whose activity is required for cell cycle G1/S transition, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle, The protein encoded by this gene belongs to the highly conserved cyclin family
Purification methodThis antibody was purified via Protein A
Storage conditions 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA
Excitation Emission494nm/518nm
Synonyms CYCLE, Ccne1, G1/S-specific cyclin-E1, Ccne
Properties Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°
Gene targetCyclin E Conjugated
Short name FITC Conjugated, Cyclin E Antibody
Technique Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody
LabelFITC
Alternative name fluorecein coupled, Cyclin E (Antibody to)
Alternative techniqueantibodies

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