| Long name | DDX58 Primary Polyclonal Antibody |
| Also known as | DDX58 PAb |
| Category | Primary Antibodies |
| Conjugation | Unconjugated |
| Target Antigen | DDX58 |
| Specificity | This is a highly specific antibody against DDX58 |
| Modification(s) | None |
| Modification site(s) | Unmodified antibody |
| Clonality | Polyclonal antibody |
| Clone number | Polyclonal antibody |
| Concentration | 1ug per 1ul |
| Subcellular location | Cytoplasm |
| Source | This antibody was obtained by immunization of the host with KLH conjugated synthetic peptide derived from human DDX58 |
| Gene ID number | 23586 |
| Swiss Prot | O95786 |
| Tested Applications | IF(IHC-P), IHC-P, WB |
| Recommended dilutions | IF(IHC-P)(1:50-200), IHC-P(1:100-500), WB(1:100-1000) |
| Cross reactivity | Mouse, Rat, Human |
| Cross reactive species details | not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Background of the target antigen | A 3'overhang at the 5'triphosphate end decreases and any 5'overhang at the 5' triphosphate end abolishes its activity, Also involved in antiviral signaling in response to viruses containing a dsDNA genome such as Epstein-Barr virus (EBV), Detects both positive and negative strand RNA viruses including members of the families Paramyxoviridae: Human respiratory syncytial virus and measles virus (MeV), Detects dsRNA produced from non-self dsDNA by RNA polymerase III, Flaviviridae: Japanese encephalitis virus (JEV), IFN-alpha and IFN-beta, In addition to the 5'-triphosphate moiety, It also detects rotavirus and reovirus, Its ligands include: 5'-triphosphorylated ssRNA and dsRNA and short dsRNA (<, May play important roles in granulocyte production and differentiation, Orthomyxoviridae: influenza A and B virus, Overhangs at the non-triphosphorylated end of the dsRNA RNA have no major impact on its activity, Rhabdoviridae: vesicular stomatitis virus (VSV), Upon ligand binding it associates with mitochondria antiviral signaling protein (MAVS/IPS1) which activates the IKK-related kinases: TBK1 and IKBKE which phosphorylate interferon regulatory factors: IRF3 and IRF7 which in turn activate transcription of antiviral immunological genes, bacterial phagocytosis and in the regulation of cell migration, blunt-end base pairing at the 5'-end of the RNA is very essential, dengue virus (DENV) and west Nile virus (WNV), hepatitis C virus (HCV), including interferons (IFNs), such as Epstein-Barr virus-encoded RNAs (EBERs), 1 kb in length), Innate immune receptor which acts as a cytoplasmic sensor of viral nucleic acids and plays a major role in sensing viral infection and in the activation of a cascade of antiviral responses including the induction of type I interferons and proinflammatory cytokines |
| Purification method | This antibody was purified via Protein A |
| Storage conditions | 50% glycerol and 0, Store at -20°, 09% sodium azide, C for up to 1 year, Keep the antibody in aqueous buffered solution containing 1% BSA |
| Synonym names | DDX58, DEAD box protein 58, Probable ATP-dependent RNA helicase DDX58, RIG-1, RIG-I, RIG-I-like receptor 1, RLR-1, Retinoic acid-inducible gene 1 protein, Retinoic acid-inducible gene I protein, SGMRT2, RIGI |
| Properties | C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Unconjugated Antibodies they should be stored frozen at - 24° |
| Gene target | DDX58 |
| Short name | DDX58 Antibody |
| Technique | antibodies against human proteins, antibodies for, Antibody |
| Alternative name | DEAD (L-aspartic acid-L-gutamic acid-alanine-L-aspartic acid) box polypeptide 58 (Antibody to) |
| Alternative technique | antibodies |
| Alternative to gene target | Cell surfaces, DDX58 and IDBG-55854 and ENSG00000107201 and 23586, DDX58 and IDBG-629773 and ENSBTAG00000003366 and 504760, Ddx58 and IDBG-135957 and ENSMUSG00000040296 and 230073, RIG-I and RIGI and RLR-1, acting on acid anhydrides, acting on acid anhydrides and also this GO :0042802: identical protein binding, acting on acid anhydrides and molecular function this GO :0030334 and regulation of cell migration and biological process this GO :0032480 and negative regulation of type I interferon production and biological process this GO :0032587 and ruffle membrane and cellular component this GO :0032727 and positive regulation of interferon-alpha production and biological process this GO :0032728 and positive regulation of interferon-beta production and biological process this GO :0034344 and regulation of type III interferon production and biological process this GO :0039528 and cytoplasmic pattern recognition receptor signaling pathway in response to virus and biological process this GO :0039529 and RIG-I signaling pathway and biological process this GO :0042802 and identical protein binding and molecular function this GO :0042993 and positive regulation of transcription factor import into nucleus and biological process this GO :0043330 and response to exogenous dsRNA and biological process this GO :0045087 and innate immune response and biological process this GO :0045944 and positive regulation of transcription from RNA polymerase II promoter and biological process this GO :0051091 and positive regulation of sequence-specific DNA binding transcription factor activity and biological process, hydrolase activity, this GO :0002230 and positive regulation of defense response to virus by host and biological process this GO :0003676 and nucleic acid binding and molecular function this GO :0003677 and DNA binding and molecular function this GO :0003690 and double-stranded DNA binding and molecular function this GO :0003725 and double-stranded RNA binding and molecular function this GO :0003727 and single-stranded RNA binding and molecular function this GO :0004386 and helicase activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005524 and ATP binding and molecular function this GO :0005737 and cytoplasm and cellular component this GO :0005829 and cytosol and cellular component this GO :0005923 and tight junction and cellular component this GO :0008152 and metabolic process and biological process this GO :0008270 and zinc ion binding and molecular function this GO :0009597 and detection of virus and biological process this GO :0009615 and response to virus and biological process this GO :0015629 and actin cytoskeleton and cellular component this GO :0016032 and viral process and biological process this GO :0016787 and hydrolase activity and molecular function this GO :0016817 and hydrolase activity, this GO :0003676: nucleic acid binding, this GO :0003676: nucleic acid binding and also this GO :0003677: DNA binding and also this GO :0003690: double-stranded DNA binding and also this GO :0003725: double-stranded RNA binding and also this GO :0003727: single-stranded RNA binding and also this GO :0004386: helicase activity and also this GO :0005515: protein binding and also this GO :0005524: ATP binding and also this GO :0008270: zinc ion binding and also this GO :0016787: hydrolase activity and also this GO :0016817: hydrolase activity, this GO :0003677: DNA binding, this GO :0003690: double-stranded DNA binding, this GO :0003725: double-stranded RNA binding, this GO :0003727: single-stranded RNA binding, this GO :0004386: helicase activity, this GO :0005515: protein binding, this GO :0005524: ATP binding, this GO :0008270: zinc ion binding, this GO :0016787: hydrolase activity, this GO :0016817: hydrolase activity, this GO :0042802: identical protein binding, DEAD (Asp-Glu-Ala-Asp) box polypeptide 58 |
| Identity | 19102 |
| Gene | DDX58 |
| Long gene name | DExD/H-box helicase 58 |
| Synonyms gene name | DEAD (Asp-Glu-Ala-Asp) box polypeptide 58 |
| Synonyms | RIG-I FLJ13599 DKFZp434J1111 |
| Synonyms name | RNA helicase RIG-I retinoic acid inducible gene I |
| Locus | 9p21, 1 |
| Discovery year | 2004-05-10 |
| GenBank acession | AF038963 |
| Entrez gene record | 23586 |
| Pubmed identfication | 21690088 |
| RefSeq identity | NM_014314 |
| Classification | DEAD-box helicases |
| Havana BLAST/BLAT | OTTHUMG00000019746 |
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