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DNA polymerase alpha Antibody

DNA polymerase alpha Antibody size: 0.1ml 263

Supplier BIOSS PRIMARY UNCONJUGATED ANTIBODIES
Price 263
Size 0.1ml
Contact us Ask Component cell markers and DNA bp. markers are genetic markerser genes or DNA sequenceswith selection audit
Long nameDNA polymerase alpha Primary Polyclonal Antibody
Also known asDNA polymerase alpha PAb
CategoryPrimary Antibodies
ConjugationUnconjugated
Target AntigenDNA polymerase alpha
SpecificityThis is a highly specific antibody against DNA polymerase alpha
Modification(s)None
Modification site(s)Unmodified antibody
ClonalityPolyclonal antibody
Clone numberPolyclonal antibody
Concentration1ug per 1ul
SourceThis antibody was obtained by immunization of the host with KLH conjugated synthetic peptide derived from human DPOLA/DNA polymerase alpha
Gene ID number5422
Tested Applications IF(IHC-P), IHC-P, WB
Recommended dilutions IF(IHC-P)(1:50-200), IHC-P(1:100-500), WB(1:100-1000)
Cross reactivity Mouse, Rat, Human
Cross reactive species details not every possible cross-reactivity is known, Due to limited amount of testing and knowledge
Background of the target antigen During the S phase of the cell cycle, The primase subunit of the polymerase alpha complex initiates DNA synthesis by oligomerising short RNA primers on both leading and lagging strands, The reason this transfer occurs is because the polymerase alpha has limited processivity and lacks intrinsic 3' exonuclease activity for proofreading error, These primers are initially extended by the polymerase alpha catalytic subunit and subsequently transferred to polymerase delta and polymerase epsilon for processive synthesis on the lagging and leading strand, a regulatory subunit POLA2/p70 and two primase subunits PRIM1/p49 and PRIM2/p58) is recruited to DNA at the replicative forks via direct interactions with MCM10 and WDHD1, and therefore is not well suited for replicating long complexes, respectively, the DNA polymerase alpha complex (composed of a catalytic subunit POLA1/p180, Plays an essential role in the initiation of DNA replication
Purification methodThis antibody was purified via Protein A
Storage conditions 50% glycerol and 0, Store at -20°, 09% sodium azide, C for up to 1 year, Keep the antibody in aqueous buffered solution containing 1% BSA
Synonym names DNA polymerase alpha catalytic subunit, DNA polymerase alpha catalytic subunit p180, DNA polymerase alpha p180 subunit, DPOLA_HUMAN, P180, POLA, POLA1, Polymerase DNA directed, Polymerase DNA-directed, alpha 1, alpha 70kD, catalytic subunit, p180 subunit, DNA polymerase alpha 1 catalytic subunit
PropertiesC, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Unconjugated Antibodies they should be stored frozen at - 24°
Gene targetDNA polymerase alpha
Short nameDNA polymerase alpha Antibody
Technique antibodies against human proteins, antibodies for, Antibody
Alternative nameDesoxyribonucleic acid polymerizing enzyme a (Antibody to)
Alternative techniqueantibodies
Identity 30073
Gene POLA2
Long gene name DNA polymerase alpha 2, accessory subunit
Synonyms gene name accessory subunit , alpha 2 (70kD subunit) polymerase (DNA) alpha 2, polymerase (DNA directed)
Synonyms FLJ21662
Synonyms name DNA polymerase alpha subunit B DNA polymerase alpha 70 kDa subunit
Locus 11q13, 1
Discovery year 2005-01-10
GenBank acession BC002990
Entrez gene record 23649
Pubmed identfication 8223465 11433027
RefSeq identity NM_002689
Classification DNA polymerases
Havana BLAST/BLAT OTTHUMG00000165951

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