Name : E2F3 Antibody, Biotin Conjugated
Supplier : BIOSS PRIMARY CONJUGATED ANTIBODIES 1
Price :333
SKU : GEN4815166881
| Long name | Biotin Conjugated, E2F3 Polyclonal Antibody |
| Category | Conjugated Primary Antibodies |
| Conjugation | Biotinylated |
| Host Organism | Rabbit (Oryctolagus cuniculus) |
| Target Antigen | E2F3 |
| Specificity | This is a highly specific antibody against E2F3 |
| Modification | Unmodified |
| Modification site | None |
| Clonality | Polyclonal |
| Clone | Polyclonal antibody |
| Concentration | 1ug per 1ul |
| Immunogen_range | 420-465/465 |
| Subcellular location | Nucleus |
| Source | KLH conjugated synthetic peptide derived from human E2F3 |
| Gene ID number | 1871 |
| Swiss Prot | O00716 |
| Tested applications | IHC-P, WB |
| Recommended dilutions | IHC-P(1:100-500), WB(1:100-1000) |
| Crossreactivity | Mouse, Rat, Human |
| Crossreactive species details | not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background | 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication, E2F3 binds specifically to RB1 in a cell-cycle dependent manner, Inhibits adipogenesis, The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase, probably through the repression of CEBPA binding to its target gene promoters (By similarity), Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site |
| Purification method | This antibody was purified via Protein A |
| Storage conditions | 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission | N/A |
| Properties | Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, Biotin conjugates can be detected by horseradish peroxidase, To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, alkaline phosphatase substrates or anti biotin conjugated antibodies, 6, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target | E2F3 Conjugated |
| Short name | Conjugated, E2F3 Antibody |
| Technique | antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label | Biotin |
| Alternative name | biotinilated coupled, E2F3 (Antibody to) |
| Alternative technique | antibodies |
| Identity | 3115 |
| Gene | E2F3 |
| Long gene name | E2F transcription factor 3 |
| Locus | 6p22, 3 |
| Discovery year | 1994-08-31 |
| GenBank acession | Y10479 |
| Entrez gene record | 1871 |
| Pubmed identfication | 8246996 |
| Classification | E2F transcription factors |
| Havana BLAST/BLAT | OTTHUMG00000016389 |