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E2F3 Antibody, FITC Conjugated

E2F3 Antibody, FITC Conjugated size: 0.1ml 333

Supplier BIOSS PRIMARY CONJUGATED ANTIBODIES 1
Price 333
Size 0.1ml
Contact us Ask Component cell markers and DNA bp. markers are genetic markerser genes or DNA sequenceswith selection audit
Long name FITC Conjugated, E2F3 Polyclonal Antibody
CategoryConjugated Primary Antibodies
ConjugationAnti-FITC Antibody
Host OrganismRabbit (Oryctolagus cuniculus)
Target AntigenE2F3
SpecificityThis is a highly specific antibody against E2F3
ModificationUnmodified
Modification siteNone
ClonalityPolyclonal
ClonePolyclonal antibody
Concentration1ug per 1ul
Immunogen_range420-465/465
Subcellular locationNucleus
SourceKLH conjugated synthetic peptide derived from human E2F3
Gene ID number1871
Swiss ProtO00716
Tested applicationsIF(IHC-P)
Recommended dilutionsIF(IHC-P)(1:50-200)
Crossreactivity Mouse, Rat, Human
Crossreactive species details not every possible cross-reactivity is known, Due to limited amount of testing and knowledge
Antigen background 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication, E2F3 binds specifically to RB1 in a cell-cycle dependent manner, Inhibits adipogenesis, The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase, probably through the repression of CEBPA binding to its target gene promoters (By similarity), Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site
Purification methodThis antibody was purified via Protein A
Storage conditions 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA
Excitation Emission494nm/518nm
Properties Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°
Gene targetE2F3 Conjugated
Short name FITC Conjugated, E2F3 Antibody
Technique Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody
LabelFITC
Alternative name fluorecein coupled, E2F3 (Antibody to)
Alternative techniqueantibodies
Identity 3115
Gene E2F3
Long gene name E2F transcription factor 3
Locus 6p22, 3
Discovery year 1994-08-31
GenBank acession Y10479
Entrez gene record 1871
Pubmed identfication 8246996
Classification E2F transcription factors
Havana BLAST/BLAT OTTHUMG00000016389

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