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EGFRvIII Antibody, Biotin Conjugated

EGFRvIII Antibody, Biotin Conjugated size: 0.1ml 333

Supplier BIOSS PRIMARY CONJUGATED ANTIBODIES 1
Price 333
Size 0.1ml
Contact us Ask Component cell markers and DNA bp. markers are genetic markerser genes or DNA sequenceswith selection audit
Long name Biotin Conjugated, EGFRvIII Polyclonal Antibody
CategoryConjugated Primary Antibodies
ConjugationBiotinylated
Host OrganismRabbit (Oryctolagus cuniculus)
Target AntigenEGFRvIII
SpecificityThis is a highly specific antibody against EGFRvIII
ModificationUnmodified
Modification siteNone
ClonalityPolyclonal
ClonePolyclonal antibody
Concentration1ug per 1ul
Immunogen_range1-50/1081
Subcellular locationExtracellular
SourceKLH conjugated synthetic peptide derived from human EGFRvIII
Gene ID number1956
Swiss ProtP00533
Tested applications IHC-P, WB
Recommended dilutions IHC-P(1:100-500), WB(1:100-1000)
CrossreactivityHuman
Crossreactive species details not every possible cross-reactivity is known, Due to limited amount of testing and knowledge
Antigen background Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, Also directly phosphorylates other proteins like RGS16, Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin, BTC/betacellulin, Isoform 2 may act as an antagonist of EGF action, Known ligands include EGF, Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues, May also activate the NF-kappa-B signaling cascade, PI3 kinase-AKT, PLCgamma-PKC and STATs modules, TGFA/TGF-alpha, The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling, amphiregulin, epigen/EPGN, epiregulin/EREG and HBEGF/heparin-binding EGF, Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses
Purification methodThis antibody was purified via Protein A
Storage conditions 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA
Excitation EmissionN/A
Synonyms EGFR, ERBB1, Epidermal growth factor receptor, HER1, NISBD2, PIG61, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, mENA, ERBB
Properties Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, Biotin conjugates can be detected by horseradish peroxidase, To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, alkaline phosphatase substrates or anti biotin conjugated antibodies, 6, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°
Gene targetEGFRvIII Conjugated
Short name Conjugated, EGFRvIII Antibody
Technique antibodies against human proteins, antibodies for, antibody Conjugates, Antibody
LabelBiotin
Alternative name biotinilated coupled, EGFRvIII (Antibody to)
Alternative techniqueantibodies

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