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EGFRvIII Antibody, FITC Conjugated

EGFRvIII Antibody, FITC Conjugated size: 0.1ml 333

Supplier BIOSS PRIMARY CONJUGATED ANTIBODIES 1
Price 333
Size 0.1ml
Contact us Ask Component cell markers and DNA bp. markers are genetic markerser genes or DNA sequenceswith selection audit
Long name FITC Conjugated, EGFRvIII Polyclonal Antibody
CategoryConjugated Primary Antibodies
ConjugationAnti-FITC Antibody
Host OrganismRabbit (Oryctolagus cuniculus)
Target AntigenEGFRvIII
SpecificityThis is a highly specific antibody against EGFRvIII
ModificationUnmodified
Modification siteNone
ClonalityPolyclonal
ClonePolyclonal antibody
Concentration1ug per 1ul
Immunogen_range1-50/1081
Subcellular locationExtracellular
SourceKLH conjugated synthetic peptide derived from human EGFRvIII
Gene ID number1956
Swiss ProtP00533
Tested applicationsIF(IHC-P)
Recommended dilutionsIF(IHC-P)(1:50-200)
CrossreactivityHuman
Crossreactive species details not every possible cross-reactivity is known, Due to limited amount of testing and knowledge
Antigen background Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, Also directly phosphorylates other proteins like RGS16, Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin, BTC/betacellulin, Isoform 2 may act as an antagonist of EGF action, Known ligands include EGF, Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues, May also activate the NF-kappa-B signaling cascade, PI3 kinase-AKT, PLCgamma-PKC and STATs modules, TGFA/TGF-alpha, The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling, amphiregulin, epigen/EPGN, epiregulin/EREG and HBEGF/heparin-binding EGF, Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses
Purification methodThis antibody was purified via Protein A
Storage conditions 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA
Excitation Emission494nm/518nm
Synonyms EGFR, ERBB1, Epidermal growth factor receptor, HER1, NISBD2, PIG61, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, mENA, ERBB
Properties Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°
Gene targetEGFRvIII Conjugated
Short name FITC Conjugated, EGFRvIII Antibody
Technique Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody
LabelFITC
Alternative name fluorecein coupled, EGFRvIII (Antibody to)
Alternative techniqueantibodies

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