Name : eIF1 Antibody, FITC Conjugated
Supplier : BIOSS PRIMARY CONJUGATED ANTIBODIES 1
Price :333
SKU : GEN4611733261
| Long name | FITC Conjugated, eIF1 Polyclonal Antibody |
| Category | Conjugated Primary Antibodies |
| Conjugation | Anti-FITC Antibody |
| Host Organism | Rabbit (Oryctolagus cuniculus) |
| Target Antigen | eIF1 |
| Specificity | This is a highly specific antibody against eIF1 |
| Modification | Unmodified |
| Modification site | None |
| Clonality | Polyclonal |
| Clone | Polyclonal antibody |
| Concentration | 1ug per 1ul |
| Source | KLH conjugated synthetic peptide derived from human eIF1 |
| Gene ID number | 10209 |
| Tested applications | IF(IHC-P) |
| Recommended dilutions | IF(IHC-P)(1:50-200) |
| Crossreactivity | Mouse, Rat, Human |
| Crossreactive species details | not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background | 48S and 40S, During the scanning process, Eukaryotic translation initiation factor 1 (eIF1) is crucial for the scanning process in vitro, In addition, It is also a promotor, Translation is also initiated by the role of eIF1 in regulating the activity of ribosomal subunits 43S, along with eukar-yotic translation initiation factor 1A (eIF1A), eIF1 and eIF1A, eIF1 enables 43S ribosomal complexes to discern between cognate and near-cognate initiation codons, eIF1 is a component of a complex involved in recognition of the initiator codon, for assembly of 48S ribosomal complexes at the initiation codon of a conventional capped mRNA, function in the formation of stable 40S ribosomal preinitiation complexes, sensing the nucleotide content of initiation codons, together with eukaryotic translation initiation factor 5 (eIF5), translation is controlled at the level of polypeptide chain initiation by initiation factors, In mammalian cells |
| Purification method | This antibody was purified via Protein A |
| Storage conditions | 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission | 494nm/518nm |
| Synonyms | E1A, E1_HUMAN, Eukaryotic translation initiation factor 1, ISO1, Protein translation factor SUI1 homolog, SUI1, Sui1iso1, e1, A121 |
| Properties | Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target | eIF1 Conjugated |
| Short name | FITC Conjugated, eIF1 Antibody |
| Technique | Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label | FITC |
| Alternative name | fluorecein coupled, eIF1 (Antibody to) |
| Alternative technique | antibodies |