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eIF2 alpha subunit 1 (Ser52) Antibody, Biotin Conjugated

eIF2 alpha subunit 1 (Ser52) Antibody, Biotin Conjugated size: 0.1ml 350

Price 350
Size 0.1ml
Long name Biotin Conjugated, eIF2 alpha subunit 1 (Ser52) Antibody
CategoryConjugated Primary Antibodies
ConjugationBiotinylated
Host OrganismRabbit (Oryctolagus cuniculus)
Target AntigeneIF2 alpha subunit 1 Ser52
SpecificityThis is a highly specific antibody against eIF2 alpha subunit 1 Ser52
ModificationPhosphorylation
Modification siteSer52
ClonalityPolyclonal
ClonePolyclonal antibody
Concentration1ug per 1ul
Immunogen_range50-91/315
Subcellular locationCytoplasm
SourceKLH conjugated synthetic phosphopeptide derived from human eIF2 alpha subunit 1 around the phosphorylation site of Ser52 [EL(p-S)RR]
Gene ID number1965
Swiss ProtP05198
Tested applications IHC-P, WB
Recommended dilutions IHC-P(1:100-500), WB(1:100-1000)
Crossreactivity Mouse, Rat, Human
Crossreactive species details not every possible cross-reactivity is known, Due to limited amount of testing and knowledge
Antigen background In order for eIF-2 to recycle and catalyze another round of initiation, Junction of the 6S ribosomal subunit to form the 8S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex, This complex binds to a 4S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B, Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA
Purification methodThis antibody was purified via Protein A
Storage conditions 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA
Excitation EmissionN/A
Synonyms EIF-2, EIF-2A, EIF-2alpha, EIF2A, EIF2S1, Eukaryotic translation initiation factor 2 subunit 1, Eukaryotic translation initiation factor 2 subunit alpha, eIF-2-alpha, EIF2
Properties Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, Biotin conjugates can be detected by horseradish peroxidase, To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, alkaline phosphatase substrates or anti biotin conjugated antibodies, 6, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°
Gene targeteIF2 alpha subunit 1 (Ser52) Conjugated
Short name Conjugated, eIF2 alpha subunit 1 (Ser52) Antibody
Technique antibodies against human proteins, antibodies for, antibody Conjugates, Antibody
LabelBiotin
Alternative name biotinilated coupled, eIF2 a functionnal sequence 1 (Ser52) (Antibody to)
Alternative techniqueantibodies

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