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eIF2 alpha subunit 1 (Ser52) Antibody, FITC Conjugated

eIF2 alpha subunit 1 (Ser52) Antibody, FITC Conjugated size: 0.1ml 350

Price 350
Size 0.1ml
Long name FITC Conjugated, eIF2 alpha subunit 1 (Ser52) Antibody
CategoryConjugated Primary Antibodies
ConjugationAnti-FITC Antibody
Host OrganismRabbit (Oryctolagus cuniculus)
Target AntigeneIF2 alpha subunit 1 Ser52
SpecificityThis is a highly specific antibody against eIF2 alpha subunit 1 Ser52
ModificationPhosphorylation
Modification siteSer52
ClonalityPolyclonal
ClonePolyclonal antibody
Concentration1ug per 1ul
Immunogen_range50-91/315
Subcellular locationCytoplasm
SourceKLH conjugated synthetic phosphopeptide derived from human eIF2 alpha subunit 1 around the phosphorylation site of Ser52 [EL(p-S)RR]
Gene ID number1965
Swiss ProtP05198
Tested applicationsIF(IHC-P)
Recommended dilutionsIF(IHC-P)(1:50-200)
Crossreactivity Mouse, Rat, Human
Crossreactive species details not every possible cross-reactivity is known, Due to limited amount of testing and knowledge
Antigen background In order for eIF-2 to recycle and catalyze another round of initiation, Junction of the 6S ribosomal subunit to form the 8S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex, This complex binds to a 4S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B, Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA
Purification methodThis antibody was purified via Protein A
Storage conditions 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA
Excitation Emission494nm/518nm
Synonyms EIF-2, EIF-2A, EIF-2alpha, EIF2A, EIF2S1, Eukaryotic translation initiation factor 2 subunit 1, Eukaryotic translation initiation factor 2 subunit alpha, eIF-2-alpha, EIF2
Properties Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°
Gene targeteIF2 alpha subunit 1 (Ser52) Conjugated
Short name FITC Conjugated, eIF2 alpha subunit 1 (Ser52) Antibody
Technique Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody
LabelFITC
Alternative name fluorecein coupled, eIF2 a functionnal sequence 1 (Ser52) (Antibody to)
Alternative techniqueantibodies

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