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eIF4E Antibody, Biotin Conjugated

eIF4E Antibody, Biotin Conjugated size: 0.1ml 333

Supplier BIOSS PRIMARY CONJUGATED ANTIBODIES 1
Price 333
Size 0.1ml
Contact us Ask Component cell markers and DNA bp. markers are genetic markerser genes or DNA sequenceswith selection audit
Long name Biotin Conjugated, eIF4E Polyclonal Antibody
CategoryConjugated Primary Antibodies
ConjugationBiotinylated
Host OrganismRabbit (Oryctolagus cuniculus)
Target Antigene4E
SpecificityThis is a highly specific antibody against e4E
ModificationUnmodified
Modification siteNone
ClonalityPolyclonal
ClonePolyclonal antibody
Concentration1ug per 1ul
SourceKLH conjugated synthetic peptide derived from human eIF4E
Gene ID number1977
Tested applications IHC-P, WB
Recommended dilutions IHC-P(1:100-500), WB(1:100-1000)
Crossreactivity Mouse, Rat, Human
Crossreactive species details not every possible cross-reactivity is known, Due to limited amount of testing and knowledge
Antigen background Phosphorylation also enhances the interaction of eIF4E with eIF4G, Phosphorylation of eIF4E on serine 209 regulates the affinity of this protein for the 7 methyl GTP cap and/or RNA, Several kinases are currently being investigated as potential regulators of eIF4E including PKC and/or the MAP kinase activated Mnk, a protein modulates translation of maternal mRNAs in early embryos before the onset of zygotic transcription, eIF4E also influences the overall rate of translation, eIF4E binds to the 7 methyl GTP cap structure of eukaryotic mRNAs, eIF4E phosphorylation is correlated with increased translational rate in a number of cell types, which form a complex known as eIF4F, eIF4E
Purification methodThis antibody was purified via Protein A
Storage conditions 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA
Excitation EmissionN/A
Synonyms CBP, CBP, E 4F, E4E1, E4EL1, E4F, Eukaryotic Translation Initiation Factor 4 E, Eukaryotic translation initiation factor 4E like 1, Messanger RNA Cap Binding Protein e 4E, e 4E, e 4E, e 4F 25 kDa subunit, e 4F 25 kDa subunit, e-4E, E4F
Properties Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, Biotin conjugates can be detected by horseradish peroxidase, To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, alkaline phosphatase substrates or anti biotin conjugated antibodies, 6, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°
Gene targeteIF4E Conjugated
Short name Conjugated, eIF4E Antibody
Technique antibodies against human proteins, antibodies for, antibody Conjugates, Antibody
LabelBiotin
Alternative name biotinilated coupled, eIF4E (Antibody to)
Alternative techniqueantibodies

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