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eIF4E (Ser209) Antibody, Biotin Conjugated

eIF4E (Ser209) Antibody, Biotin Conjugated size: 0.1ml 350

Price 350
Size 0.1ml
Long name Biotin Conjugated, eIF4E (Ser209) Polyclonal Antibody
CategoryConjugated Primary Antibodies
ConjugationBiotinylated
Host OrganismRabbit (Oryctolagus cuniculus)
Target AntigeneIF4E Ser209
SpecificityThis is a highly specific antibody against eIF4E Ser209
ModificationPhosphorylation
Modification siteSer209
ClonalityPolyclonal
ClonePolyclonal antibody
Concentration1ug per 1ul
Immunogen_range200-217/217
Subcellular locationHelical
SourceKLH conjugated synthetic phosphopeptide derived from human eIF4E around the phosphorylation site of Ser209 [SG(p-S)TT]
Gene ID number1977
Swiss ProtP06730
Tested applications IHC-P, WB
Recommended dilutions IHC-P(1:100-500), WB(1:100-1000)
Crossreactivity Mouse, Rat, Human
Crossreactive species details not every possible cross-reactivity is known, Due to limited amount of testing and knowledge
Antigen background Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression, In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap, Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures
Purification methodThis antibody was purified via Protein A
Storage conditions 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA
Excitation EmissionN/A
Synonyms AUTS19, EIF4E1, EIF4EL1, EIF4F, Eukaryotic translation initiation factor 4E, eIF-4E, eIF-4F 25 kDa subunit, eIF4E, mRNA cap-binding protein, CBP
Properties Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, Biotin conjugates can be detected by horseradish peroxidase, To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, alkaline phosphatase substrates or anti biotin conjugated antibodies, 6, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°
Gene targeteIF4E (Ser209) Conjugated
Short name Conjugated, eIF4E (Ser209) Antibody
Technique antibodies against human proteins, antibodies for, antibody Conjugates, Antibody
LabelBiotin
Alternative name biotinilated coupled, eIF4E (Ser209) (Antibody to)
Alternative techniqueantibodies

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