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EMP Antibody, Biotin Conjugated

EMP Antibody, Biotin Conjugated size: 0.1ml 333

Price 333
Size 0.1ml
Long name Biotin Conjugated, EMP Polyclonal Antibody
CategoryConjugated Primary Antibodies
ConjugationBiotinylated
Host OrganismRabbit (Oryctolagus cuniculus)
Target AntigenMAEA/EMP/HLC10
SpecificityThis is a highly specific antibody against MAEA/EMP/HLC10
ModificationUnmodified
Modification siteNone
ClonalityPolyclonal
ClonePolyclonal antibody
Concentration1ug per 1ul
SourceKLH conjugated synthetic peptide derived from human EMP
Gene ID number10296
Tested applications IHC-P, WB
Recommended dilutions IHC-P(1:100-500), WB(1:100-1000)
Crossreactivity Mouse, Rat, Human
Crossreactive species details not every possible cross-reactivity is known, Due to limited amount of testing and knowledge
Antigen background Although the exact function of Emp is unknown, Considered to assist with cell division and nuclear architecture, Emp can form a complex with F-actin, Emp contains one CTLH domain and one LisH domain, Emp is localized with condensed chromatin at prophase, Emp is suggested to be involvement in erythroblast-macrophage cell attachment, Expressed as 5 alternatively spliced isoforms, and inhibiting apoptosis of erythroblasts, nuclear spindle poles at metaphase and in the contractile ring during telophase and cytokinesis, terminal maturation and enucleation of erythroid cells, which is involved regulating actin distribution in erythroblasts and macrophages, Emp is a 396 amino acid ubiquitously expressed adhesion protein
Purification methodThis antibody was purified via Protein A
Storage conditions 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA
Excitation EmissionN/A
Synonyms EMP GID9
Properties Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, Biotin conjugates can be detected by horseradish peroxidase, To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, alkaline phosphatase substrates or anti biotin conjugated antibodies, 6, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°
Gene targetEMP Conjugated
Short name Conjugated, EMP Antibody
Technique antibodies against human proteins, antibodies for, antibody Conjugates, Antibody
LabelBiotin
Alternative name biotinilated coupled, EMP (Antibody to)
Alternative techniqueantibodies
Identity 13731
Gene MAEA
Long gene name macrophage erythroblast attacher
Synonyms name FYV10 homolog (S, GID complex subunit 9, cerevisiae)
Locus 4p16, 3
Discovery year 2000-11-07
GenBank acession AF084928
Entrez gene record 10296
Pubmed identfication 9763581
RefSeq identity NM_005882
Havana BLAST/BLAT OTTHUMG00000160169

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