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EN2/Engrailed 2 Antibody, FITC Conjugated

EN2/Engrailed 2 Antibody, FITC Conjugated size: 0.1ml 333

Supplier BIOSS PRIMARY CONJUGATED ANTIBODIES 1
Price 333
Size 0.1ml
Contact us Ask Component cell markers and DNA bp. markers are genetic markerser genes or DNA sequenceswith selection audit
Long name FITC Conjugated, EN2/Engrailed 2 Polyclonal Antibody
CategoryConjugated Primary Antibodies
ConjugationAnti-FITC Antibody
Host OrganismRabbit (Oryctolagus cuniculus)
Target AntigenEN2/Engrailed 2
SpecificityThis is a highly specific antibody against EN2/Engrailed 2
ModificationUnmodified
Modification siteNone
ClonalityPolyclonal
ClonePolyclonal antibody
Concentration1ug per 1ul
Immunogen_range300-333/333
Subcellular locationNucleus
SourceKLH conjugated synthetic peptide derived from human EN2/Engrailed 2
Gene ID number2020
Swiss ProtP19622
Tested applicationsIF(IHC-P)
Recommended dilutionsIF(IHC-P)(1:50-200)
Crossreactivity Mouse, Rat, Human
Crossreactive species details not every possible cross-reactivity is known, Due to limited amount of testing and knowledge
Antigen background Different mutations in the mouse homologs, During early brain development mouse EN-2 is expressed in a broad band across most of the mid-hindbrain region, EN-1 and EN-2, EN-1 and EN-2 regulate expression of a-synuclein, EN-1 is highly expressed by essentially all dopaminergic neurons in the substantia nigra and ventral tegmentum, EN-2 is also expressed in mouse myoblasts and has been assiciated with cerebellar hypoplasia, EN2, Human EN-1 and EN-2 are homeodomain-containing proteins and have been implicated in the control of pattern formation during development of the central nervous system, In Drosophila, a gene that is genetically linked to Parkinsonâ, a murine homolog of the Drosophila homeobox gene engrailed (EN), is required for midbrain and cerebellum development and dorsal/ventral patterning of the limbs as well as apical ectodermal ridge formation, produce different developmental defects that frequently are lethal, the EN gene plays an important role during development in segmentation, where it is required for the formation of posterior compartments, &euro, &trade, The engrailed-2 gene, s disease
Purification methodThis antibody was purified via Protein A
Storage conditions 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA
Excitation Emission494nm/518nm
Properties Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°
Gene targetEN2/Engrailed 2 Conjugated
Short name FITC Conjugated, EN2/Engrailed 2 Antibody
Technique Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody
LabelFITC
Alternative name fluorecein coupled, EN2/Engrailed 2 (Antibody to)
Alternative techniqueantibodies
Identity 3343
Gene EN2
Long gene name engrailed homeobox 2
Locus 7q36, 3
Discovery year 1988-08-31
Entrez gene record 2020
RefSeq identity NM_001427
Classification NKL subclass homeoboxes and pseudogenes
Havana BLAST/BLAT OTTHUMG00000151354

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